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Human Macrophage Nucleofector™ Kit
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Catalog #: VPA-1008
For efficient transfection of primary human macrophages in the NucleofectorTM I/II/2b Device
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Product Overview
The NucleofectorTM 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This NucleofectorTM Kit is the optimal kit for efficient transfectiion of primary human macrophages.
The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.
Benefits
- High transfection efficiencies for primary cells
- Cells maintain functionality post transfection
Applications
- Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells
- Low-throughput transfection in single cuvette format
Content & Storage
Content
2.25 mL NucleofectorTM Solution for Human Macrophages
0.5 mL Supplement 2
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL)
25 Single use pipettes
Instructions
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