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Human B Cell Nucleofector™ Kit

Human B Cell Nucleofector™ Kit

Catalog #: VPA-1001
For efficient transfection of primary human B cells in the NucleofectorTM I/II/2b Device
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 683.00 CHF
ST

Product Overview

The NucleofectorTM 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This NucleofectorTM Kit is the optimal kit for efficient transfectiion of primary human B cells.

The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.

 

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Benefits

  • High transfection efficiencies for primary cells
  • Cells maintain functionality post transfection

Applications

  • Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells 
  • Low-throughput transfection in single cuvette format

Storage and Content

  • 2.25 mL NucleofectorTM Solution for Human B Cells
    0.5 mL Supplement 1
    30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
    25 Aluminum cuvette (100 µL)
    25 Single use pipettes

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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • Transfection Protocol for Human B Cells - Nucleofector™ I/II/2b Device Series

    Instructions of use for transfection of human B cells with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)
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