Cell Line Nucleofector™ Kit L
Cell Line Nucleofector™ Kit L
For efficient transfection of cell lines, e.g. 3T3-L1 ad, DU145, EL4, IMR-32 or MDCK, in the NucleofectorTM I/II/2b Device
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Product Overview
The Cell Line NucleofectorTM Kit L is one of our five NucleofectorTM Kits suited for transfecting cell lines in the NucleofectorTM 2b Device.
This kit has been determined to be optimal for transfecting following cell types:
3T3-L1 ad
A-10
BHK-21
BxPC-3
DU145
EL4
FDC-P1
GH3 H9c2 (2-1)
HeLa S3
IMR-32
KG-1a
L-428 (DSMZ)
MDCK
MDCK II (ECACC)
MOLT-4
MV-4-11
NTERA-2 cl.D1
WEHI-231
The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.
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Benefits
- High transfection efficiencies in difficult-to-transfect cell lines
Applications
- Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells
- Low-throughput transfection in single cuvette format
Storage and Content
2.25 mL NucleofectorTM Solution L
0.5 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL),
25 Single use pipettes
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
