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Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Negative Selection, 50 million
Catalog #: 4W-400A
Cryopreserved ampule of Human Peripheral Blood CD14+ Monocytes, negative selection, containing ≥ 50 million cells
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Product Overview
Leukapheresed CD14+ monocytes are negatively selected from leukapheresed peripheral blood MNCs using an immunomagnetic labeling system to remove unwanted cell types leaving the monocyte untouched. Cells are cryopreserved in Lonza Cryo Media in a cryovial. Cell purity is determined post thaw using a FACS assay. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols. Manufactured by AllCells®
Benefits
- Test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis (CoA) is provided for each cell lot purchased
Applications
- Generate monocyte-derived M1 and M2 macrophages
- Generate monocyte-derived DCs
- Dendritic cell differentiation and maturation
- Toll-like receptor and pattern recognition receptors (PRR)
- Phagocytosis
- Metabolism mechanisms
Content & Storage
Content
1 x Cryopreserved ampule of CD14+ Monocytes containing ≥ 50 million cells