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Pharma & Biotech

H-RPE – Human Retinal Pigment Epithelial Cells

Catalog #: 00194987

Cryopreserved ampule of Human Retinal Pigment Epithelial Cells (H-RPE) containing ≥500,000 cells 

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Product Overview

The human retinal pigment epithelium (H-RPE) consists of pigmented cells situated between the neuroretina and the choroids. The H-RPE is comprised of hexagonal cells organized into a monolayer that is densely packed with pigment granules. The H-RPE is of the neuroectodermal origin and is therefore considered to be part of the retina. H-RPE cells play a critical role in visual function and photoreceptor viability.

Lonza's H-RPE (Human Retinal Pigment Epithelial) cells are cryopreserved, primary cells that are packaged at passage 2 and contain ≥500,000 cells per vial.  H-RPE are guaranteed through 5 population doublings and stain positive (≥90%) for pancytokeratin and Zo-1 and negative (≤10%) for fibroblast contamination and endothelial marker CD31 ( ≤1%). They also test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.

Benefits

  • Guaranteed through 5 population doublings 
  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate ofAnalysis is provided for each cell lot purchased

 

Applications

  • Age-related macular degeneration
  • Retinitis pigmentosa
  • Diabetic retinopathy
  • Photoreceptor degeneration
  • Gene expression
  • Cellular interactions

Content & Storage

Content

1 x Cryopreserved ampule of H-RPE containing ≥500,000 cells

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Structure of Retinal Epithelial Cells

The apical membrane of the RPE faces the photoreceptor’s outer segments and its basolateral membrane faces Bruch’s membrane. Bruch's membrane separates the RPE from the fenestrated endothelium of the choriocapillaris (Fig 2). The RPE constitutes the outer blood-retinal barrier (BRB). The inner BRB is mainly constituted by the endothelial cells. Tight junctions between neighboring RPE cells and neighboring endothelial cells are essential in the strict control of fluids and solutes that cross the BRB as well as in preventing the entrance of toxic molecules and plasma components into the retina.

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