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Pharma & Biotech

NHNP – Human Neural Progenitor Cells

Catalog #: PT-2599

Cryopreserved ampule of Normal Human Neural Progenitor (NHNP) cells containing ≥1.2 million cells

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Product Overview

PoieticsTM Neural Progenitor Cells (NHNP) are cryopreserved neurospheres isolated from human brain cortex. Neurospheres are clusters of cells typically referred to as neural stem cells and progenitors (NSPCs) or Human midbrain-derived neural progenitor cells (hmNPCs). These neurospheres can be maintained undifferentiated in suspension as a 3D aggregate. To induce differentiation, the neurospheres can be transferred to laminin-coated vessels with brain-derived neurotrophic (BDNF) which will induce differentiation into neurons, astrocytes, and oligodendrocytes.  

Cryopreserved PoieticsTM Human Neural Progenitors (NHNP) are guaranteed to contain ≥1.2 million viable cells per ampoule and test positive for Neuronal Class II Beta Tubulin and Glial Fibrillary Acid Protein (GFAP) following differentiation. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Recommended Medium: NPMMTM Neural Progenitor Maintenance BulletKitTM Medium for recovery from cryopreservation and maintenance as neurospheres; NPDMTM Neural Progenitor Differentiation BulletKitTM Medium + 25 ng/ml brain-derived neurotrophic factor (BDNF) for non-directed differentiation of neurospheres into neurons, astrocytes, and oligodendrocytes.

Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

Benefits

  • All cells test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis provided for every cell lot

 

Applications

  • Drug development
  • Neurotoxicity
  • Neurogenesis and CNS function
  • Neurotransmitter disorders
  • Electrophysiology 

Content & Storage

Content

1 x Cryopreserved ampule NHNP containing ≥ 1.2 million cells

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

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