X-VIVOTM 15 Serum-free Hematopoietic Cell Medium
X-VIVOTM 15 Serum-free Hematopoietic Cell Medium
X-VIVOTM 15, Serum free hematopoietic cell medium, with L-Glutamine, gentamicin and phenol red, xenofree 1 L
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Product Overview
X-VIVOTM 15 Media are similar in composition to X-VIVOTM 10 Media and have been optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. X-VIVOTM 15 Media support the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors. X-VIVOTM 15 Media can also be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells. In addition, X-VIVOTM 15 Media provide a serum-free environment for the expansion of HUT-78 and related human lymphocytic cell lines.
All BioWhittakerTM Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures. If you require GMP grade media, contact Lonza for more details.
Benefits
- Superior media for most hematopoietic cells types including dendritic cells, lymphocytes, monocytes, macrophages and granulocytes.
- Formulations only contain human derived components
- Highly cited in scientific literature
- Clinical proven formulations providing process safety and constancy
- High productivity resulting in a high viable cell yield
- Serum Free media result in a more defined process and result in limited immune response
- Efficient transgene expression
- Desirable T-cell phenotype
Applications
- Proliferation of peripheral blood lymphocytes
- Proliferation of tumor infiltrating lymphocytes
- Cryopreservation of human tissue
- Cultivation of human monocytes and macrophages
- Cultivation of stem cells
- Cultivation of dendritic cells
- Cultiviation of CAR-T cells
Storage and Content
1L bottleSDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
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References - X-Vivo™
Overview of relevant references -
Protocols for converting cells to serum-free culture
Procedures how to get away from serum. -
TechSheet - X-VIVO™ Media Systems
Instructions for how to use the X-VIVO™ Media Series -
B-ALI Immune-Airway Co-Culture Model: Normal Human Bronchial Epithelial cells and Peripheral Blood Mononuclear Cells - Instructions for use
Protocol to culture B-ALI NHBE cells with PBMCs
Educational Material
Brochures, White Papers etc.
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Developing Patient-specific Cell Therapy Manufacturing Processes
Poster presentation about patient-specific cellular therapies and their tremendous potential for being able to treat life threatening diseases where there are currently no cures. -
CAR T cells in Cancer Therapy
A poster developed by Nature Reviews in conjunction with Lonza to present CAR T cells in cancer therapy. This poster demonstrates the recognition of tumor peptides by T cells, CAR T cell manufacturing, the types of chimeric antigen receptors and a timeline of CAR development over more than 30 years. -
A Non-Viral Method for Modifying and Expanding Primary T Cells
Learn how Lonza’s Cocoon® Platform, 4D-Nucleofector® LV Unit and X-VIVO® 15 Medium are integrated to enable non-viral genetic modification and subsequent expansion of primary T cells in a functionally-closed, automated workflow. -
PBMC Based T Cell Proliferation Assay
Protocol instructions