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Pharma & Biotech

PNGMTM Primary Neuron Growth Medium SingleQuotsTM Supplements and Growth Factors

Catalog #: CC-4462

PNGMTM Primary Neuron Growth Medium SingleQuotsTM Supplements and Growth Factors (CC-4462) contains all growth factors and supplements required for successful culture of our rat and mouse neurons.  

Please note:  PNGMTM SingleQuotsTM Kit (CC-4462) contains growth supplements only and does not include additional required PNBMTMBasal Media (CC-3652).

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Product Overview

PNGMTM Primary Neuron Growth Medium SingleQuotsTM  Supplements and Growth Factors were specifically developed for culturing embryonic primary rat and mouse neurons. For successful culture of neurons, growth factors and supplements should be combined with PNBMTM Basal Medium (CC-3256) (sold seperately).

PNBMTM Basal medium (CC-3256) and Singlequot Growth Factors (CC-4462) can be purchased seperately or in convenient PNGMTM BulletKitTM format (CC-4461)

Benefits

  • Serum-free
  • Allows for long-term maintenance and growth of neuronal cells
  • Promotes development of high quality neuronal networks without the need of an astrocyte feeder layer

 

 

Content & Storage

Content

  • 1 x NSF-1, 4.0 mL 
  • 1 x L-Glutamine, 2.0 mL 
  • 1 x GA, 0.2 mL

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

ViaLightPlus Assay in PNGMTM Media of Rat Cortical Neurons

Comparison of survival of CloneticsTM Primary Rat Cortical Neurons cultured in PNGMTM Medium vs. Company A’s medium. Cryopreserved cells were thawed and plated in laminin-coated, 96 well plates at ~1000 cells per square mm (40% of the recommended seeding density) using either PNGMTM Medium or Company A’s medium. Cells were cultured without feeding for six days, at which time cell viability was assessed using the ViaLightTM Plus Cell Proliferation and Cytotoxicity BioAssay Kit. Results represent the average of 8 experiments.

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