Cell Line Optimization 96-well Nucleofector® Kit
For rapid determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector® 96-well Unit.
What You May Also Need
Product Overview
The Cell Line Optimization 96-well Nucleofector® Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector® 96-well Unit.
With the unique capability of the 4D-Nucleofector® 96-well Unit to address individual wells of a 96-well NucleocuvetteTM Plate with different programs, primary cell optimizations are easily performed within one experiment. Using one 96-well Nucleocuvette® Plate and our pmaxGFPTM Vector as positive control, our three Cell Line Nucleofector® Solutions SE, SF and SG are tested in combination with a pre-selected set of programs plus controls.
Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.
Benefits
- Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
- Optimal Nucleofection conditions determined on the 4D-Nucleofector® 96-well Unit are transferable to the 4D-Nucleofector® System or the 384-well HT Nucleofector® System
Applications
- Determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector® 96-well Unit
Content & Storage
Content
0.675 mL Nucleofector® Solution SE
0.675 mL Nucleofector® Solution SF
0.675 mL Nucleofector® Solution SG
3 x 0.15 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
1 x 96-well Nucleocuvette® Plate
