What the RAFT™ System Consists of

RAFT^TM Reagent Kit – rat tail collagen 1 as the extracellular matrix scaffold and a specially-designed neutralizing solution that enables neutralization to be carried out in a single step instead of an error-prone and tedious titration method. The kit also contains 10X MEM to support cells through the RAFT^TM Process.

Novel RAFT^TM Absorbers – The biocompatible absorbers are designed to work with standard 96- or 24-well cell culture plates as well as 24-well cell culture inserts. Once the cells are mixed with RAFT^TM Reagents, the absorbers are utilized to compress and absorb excess liquid from the collagen mixture. This results in the creation of well-controlled, high-density collagen scaffolds embedded with cell type(s) of choice. The RAFT^TM System is one of the few commercially available kits that allows users to create these high-density collagen scaffolds that are representative of a natural, more in vivo like environment for cells to grow and interact more efficiently.

Formats of the RAFT^TM System

RAFT^TM 24-well Insert Kit for Cell Culture Inserts and Air-Lift Models

RAFT^TM Insert Absorbers are designed to be used with 24-well permeable membrane cell culture inserts. This additional kit is offered to enhance the versatility of the system further by allowing researchers to generate co-culture models, and barrier models including air-lift models.

Please note no cell culture inserts are supplied within the RAFT^TM 24-well Insert Kit due to variety in pore size. Please contact Scientific Support for a detailed list of RAFT^TM Kit compatible inserts or visit the FAQ section in the Lonza Knowledge Center.

RAFT^TM24-well Kit for Single or Co-culture Models

The RAFT^TM 24-well Kit is designed to be used for performing co-cultures or single cultures in a 24-well plate.

Please note no 24-well plates are supplied with the kit.

RAFT^TM 96-well Kit for high-throughput screening in a semi-automated manner

The RAFT^TM 96-well Kit is designed for high throughput screening. The absorbers are molded in a plate offering a semi-automated process of creating 96 x tissue-like 3D cultures within a span of few minutes.

Please contact Scientific Support for application data on how the RAFT^TM 96-well System has been automated for drug discovery applications with leading manufacturers’ liquid handling systems.

How to Setup the RAFT^TM System

Watch the video and download the PDF below to get a better understanding of how the RAFT^TM System works and how you can create 3d cultures using one or more cell types.

RAFT™ 3D Cell Culture System – How it Works

The novel RAFT™ 3D Cell Culture System is designed to closely mimic the native environment of cells. The RAFT™ 3D Cell Culture Kit offers a complete solution with the necessary reagents and consumables to develop RAFT™ 3D Cultures.

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Setting up RAFT™ 3D Cell Cultures with one or more cell types

This document shows many ways how RAFT™ 3D Culture System can be setup with single cell type or more.

Buy a trial kit today in one of these formats - 24-well, 24-well cell culture insert, 96-well.

Any questions? You wonder if the RAFT^TM System is applicable to your project? Contact us to get support.

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What the RAFTTM System Consists of

Formats of the RAFTTM System

How to Setup the RAFTTM System

RAFT™ 3D Cell Culture System – How it Works

Setting up RAFT™ 3D Cell Cultures with one or more cell types

What the RAFT^TM System Consists of

Formats of the RAFT^TM System

How to Setup the RAFT^TM System