RAFTTM Reagent Kit – rat tail collagen 1 as the extracellular matrix scaffold and a specially-designed neutralizing solution that enables neutralization to be carried out in a single step instead of an error-prone and tedious titration method. The kit also contains 10X MEM to support cells through the RAFTTM Process. 

 

Novel RAFTTM Absorbers – The biocompatible absorbers are designed to work with standard 96- or 24-well cell culture plates as well as 24-well cell culture inserts. Once the cells are mixed with RAFTTM Reagents, the absorbers are utilized to compress and absorb excess liquid from the collagen mixture. This results in the creation of well-controlled, high-density collagen scaffolds embedded with cell type(s) of choice. The RAFTTM System is one of the few commercially available kits that allows users to create these high-density collagen scaffolds that are representative of a natural, more in vivo like environment for cells to grow and interact more efficiently.

 

 

Formats of the RAFTTM System

RAFTTM 24-well Insert Kit for Cell Culture Inserts and Air-Lift Models

RAFTTM Insert Absorbers are designed to be used with 24-well permeable membrane cell culture inserts. This additional kit is offered to enhance the versatility of the system further by allowing researchers to generate co-culture models, and barrier models including air-lift models. 


Please note no cell culture inserts are supplied within the RAFTTM 24-well Insert Kit due to variety in pore size. Please contact Scientific Support for a detailed list of RAFTTM Kit compatible inserts or visit the FAQ section in the Lonza Knowledge Center.

RAFTTM24-well Kit for Single or Co-culture Models

The RAFTTM 24-well Kit is designed to be used for performing co-cultures or single cultures in a 24-well plate.

Please note no 24-well plates are supplied with the kit.

RAFTTM 96-well Kit for high-throughput screening in a semi-automated manner

The RAFTTM 96-well Kit is designed for high throughput screening. The absorbers are molded in a plate offering a semi-automated process of creating 96 x tissue-like 3D cultures within a span of few minutes.

Please contact Scientific Support for application data on how the RAFTTM 96-well System has been automated for drug discovery applications with leading manufacturers’ liquid handling systems.

How to Setup the RAFTTM System

Watch the video and download the PDF below to get a better understanding of how the RAFTTM System works and how you can create 3d cultures using one or more cell types. 

RAFT™ 3D Cell Culture System – How it Works

The novel RAFT™ 3D Cell Culture System is designed to closely mimic the native environment of cells. The RAFT™ 3D Cell Culture Kit offers a complete solution with the necessary reagents and consumables to develop RAFT™ 3D Cultures.
Download Document

Setting up RAFT™ 3D Cell Cultures with one or more cell types

This document shows many ways how RAFT™ 3D Culture System can be setup with single cell type or more.
Download Document

Buy a trial kit today in one of these formats -  24-well, 24-well cell culture insert, 96-well.

 

Any questions? You wonder if the RAFTTM System is applicable to your project? Contact us to get support.

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