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346 Documents found
  • Nucleofector™ Technology – Guideline for Easy Set Up of RNAi Screening Experiments
    This guideline aims to help researchers in setting up a successful RNAiscreening experiment using the Nucleofector™ 96-well Shuttle™ System. The recommendations are based on our experience gained in using theNucleofector™ 96-well Shuttle™ System for screens with Thermo ScientificsiRNA libraries in difficult-to-transfect Jurkat T cells and primary HUVECcells2, combined with recommendations collected from the literature. It highlights important parameters that may influence the quality of cellbasedscreening results.
  • Adherent Nucleofection™ of Cryopreserved Rat DRGs
    The White Paper shows results for transfecting cultured Lonza cryopreserved rat DRG neurons using the 4D-Nucleofector Y Unit (and adherent Nucleocuvette Strips).
  • Transfection of Human Induced Pluripotent Stem Cells using Nucleofector® Technology
    Technical Reference Guide providing tips for transfecting iPSCs using the Nucleofector® Technology
  • the next path to cell and gene therapy success…
    Brochure showcasing Lonza products and servies from discovery to commercialization
  • RT-PP190_Prepare-Lab-of-the-Future
    RT-PP190_Prepare-Lab-of-the-Future_Final-sec.pdf
  • Preparing for The QC Lab of the Future
    Streamline testing workflows with integrated lab automation.
  • MODA® Nephron Case Study - Developing an Electronic Batch Record Solution That Meets Pharmaceutical Manufacturers’ Needs
    How Lonza worked with pharmaceutical manufacturer Nephron Pharmaceuticals to develop and implement the MODA-ES® Platform in an accelerated timeframe.
  • Non-viral Gene Modification for Ex-vivo Cell Therapy
    In this interview our Director Transfection R&D discusses the advantages of non-viral transfection technologies for CRISPR- or transposon-based genetic modification cell and gene therapy.
  • First siRNA Library Screening in Hard-to-Transfect HUVEC and Jurkat Cells
    High throughput transfection of siRNA libraries has become a valuable tool in target identifi cation and validation. However, such screenings have so far been constrained to mostly easy-totransfect adherent cell lines. Lonza’s 96-well Shuttle® System, based on the well-established Amaxa® Nucleofector® Technology, extends these approaches to primary and diffi cult-to-transfect cells.
  • SP2/0 Transfection Protocol - 4D-Nucleofector™ Device
    Instructions of use for transfection of SP2/0 cells with the SF Cell Line 4D-Nucleofector™ X Kit
  • EL4 Transfection Protocol - 4D-Nucleofector™ Device
    Instructions of use for transfection of EL4 cells with the SF Cell Line 4D-Nucleofector™ X Kit
  • HL-60 Transfection Protocol - 4D-Nucleofector™ Device
    Instructions of use for transfection of HL-60 cells with the SF Cell Line 4D-Nucleofector™ X Kit
  • MCF7 Transfection Protocol - 4D-Nucleofector™ Device
    Instructions of use for transfection of MCF7 with the SE Cell Line 4D-Nucleofector™ X Kit
  • RAW 264.7 Transfection Protocol - 4D-Nucleofector™ Device
    Instructions of use for transfection of RAW 264.7 cells with the SF Cell Line 4D-Nucleofector™ X Kit
  • Instructions - PYROSPERSE® Dispersing Agent (English)
    Detailed instructions for using PYROSPERSE® Dispersing Agent.
  • 4D-Nucleofector™ Protocol for CCRF-CEM Cells
    Instructions for CCRF-CEM Cells for 4D-Nucleofector™ X Unit – Transfection Suspension
  • CHO-S (Invitrogen) Transfection Protocol - 4D-Nucleofector™ X-Unit
    Instructions of use for transfection of CHO-S cells (Invitrogen) with the SF Cell Line 4D-Nucleofector™ X Kit
  • A20 Transfection Protocol - 4D-Nucleofector™ X-Unit
    Instructions of use for transfection of A20 cells with the SF Cell Line 4D-Nucleofector™ X Kit
  • L428 Transfection Protocol - 4D-Nucleofector™ X-Unit
    Instructions of use for transfection of L428 cells with the SF Cell Line 4D-Nucleofector™ X Kit
  • NSO Transfection Protocol - 4D-Nucleofector™ X-Unit
    Instructions of use for transfection of NSO cells with the SF Cell Line 4D-Nucleofector™ X Kit