Std ProMDCK (2D)

Product Overview

Product Information

ProMDCK@&rade is a serum free media dedicated to the growth and infection of Madin-Darby Kidney (MDCK) cells in all conditions, produced GMP and labeled for further manufacturing use.

Background

MDCK cells are heteroploid, epithelial like, adherent cells originally isolated from the kidney of a normal adult female cocker spaniel. They have been used in functional cell biology studies such as the mechanisms of passive salt, protein, lipid and drug transports but also in in vitro growth regulation and cytotoxicity studies. In bioprocessing, MDCK cells have been classically used for viral vaccine manufacturing. While MDCK cells have been strongly recommended by the world health organization (WHO) in the isolation of influenza A and influenza B viruses (mainly for diagnosis purpose) they are actually a cell line of choice for producing seasonal or pandemic Influenza vaccines on cells instead of cultivation on eggs. Indeed ProMDCK&trade media has been optimized for both MDCK cell expansion and virus infection in planar culture (2D version) and on microcarriers in stirred bioreactors (3D version). Both versions of this media have been demonstrated to support cell growth and expansion of influenza virus (H1N1, H3N2, and B) allowing all upstream bioprocessing steps with one single medium. Features Comment Serum-free yes Non-animal origin Yes Protein free No Antibiotic free Yes L-glutamine no Supplementation required before use Storage temperature 2-8C Shelf life 18 months from date of manufacture