Trypsin 1:250 (10X)
Trypsin (17-160) is a clear, colorless-yellow, highly active porcine enzyme preparation, used to remove cells from the culture vessel surface into suspension.
It is generally used at a working concentration of 0.25%, prepared in a balanced salt solution, without calcium or magnesium at a pH of 7.6-7.8.
After treatment, trypsin activity can be halted by adding a serum media mixture or a Trypsin inhibitor (from soybean, for an example) in serum-free systems.
Content & Storage
100 ml bottle