Trypsin-Versene® (17-161) or Trypsin-EDTA (ethylenediaminetetraacetic acid) is a combined reagent used to remove cells from the culture vessel surface into suspension.
The EDTA, a chelator of divalent cations, is added to trypsin solutions to enhance activity.
The calcium and magnesium in the extracellular matrix, which aids in cell-cell adhesion, also obscures the peptide bonds that trypsin acts on. The EDTA is added to remove the calcium and magnesium from the cell surface which allows trypsin to hydrolyze specific peptide bonds.
This activity can be halted by adding a serum media mixture, or a Trypsin inhibitor (from soybean, for example) in serum-free systems.
It is supplied at use dilution. 17-161 contains 1700000U\L Trypsin 1:250, 2 g/L Versene (EDTA). Porcine parvovirus tested and mycoplasma screened. Product is manufactured with irradiated trypsin. BE02-007E is 10x BE17-161E.
Content & Storage
100 ml bottle