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Pharma & Biotech

ProNS0TM 1 Serum -free Medium, Protein-free, Chemically Defined, 1L

Catalog #: 12-773Q

ProNS0TM 1 Serum -free Medium, protein free medium, chemically defined, with HEPES and Pluronic® F-68, without L-Glutamne, phenol red or cholesterol, 1 L.

This product is replaced by BP12-773Q .


Product Overview

The NS0 cell line is widely used for recombinant mammalian protein expression. BioWhittakerTM ProNS0TM 1 Serum -free Medium:

  • Optimized formula is available to cover the broadest range of NS0 nutritional needs
  • Further maximize protein production by titration with ProNS0TM Lipid CD Supplement
  • Protein-free and chemically defined formulations
  • Product purification is simplified
  • Lot-to-lot consistency ensures dependable performance
  • Non-animal origin components reduce regulatory burdens

Some of the reasons to select this mouse myeloma platform are:

  • Forms stably producing hybrid cells with high levels of protein production
  • Lacks ability to secrete endogenous antibody or antibody fragments
  • Resists aggregation clumping in suspension culture
  • Lacks adverse protease activity on recombinant protein product (in many cases)
  • BioWhittakerTMProNS0TM 1 Serum-free Medium, together with ProNS0TM Lipid CD Supplement, is designed to meet a growing demand for optimized NS0 formulations.

Titering the separate lipid supplement permits further optimization that can have a significant positive impact on protein production. Suggested starting concentration for optional addition of ProNS0TM Lipid CD Supplement is 5 ml per liter.

All BioWhittakerTM Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures. If you require GMP grade media, contact Lonza for more details.


 ProNS0TM 1 Serum -free Medium

  • High density cell culture of NS0 cells

Content & Storage


1L bottle


Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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