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  • Genome Editing using Nucleofector® Technology – Citation List
    Selected citations for genome editing using ZFN, TALEN or CRISPR/Cas9 or transposition systems (e.g. for CAR-T) in combination with Lonza's Nucleofector® Technology.
  • MDCK II Transfection Protocol - Nucleofector™ I/II/2b Device Series
    Instructions of use for transfection of MDCK II cells (ECACC) with the Cell Line Nucleofector™ Kit L
  • CHO [Suspension] Transfection Protocol - Nucleofector™ I/II/2b Device Series
    Instructions of use for transfection of suspension CHO cells (ECACC) with the Cell Line Nucleofector™ Kit V
  • A 3D Bioprinted Model to Study Osteogenic Differentiation of Primary Mesenchymal Stem Cells
    Learn from this white paper how a 3D bioprinted model using hMSCs can be used for evaluation of bioink compatibility and bioprinting processes.
  • Transfection Protocol for HUVEC - 96-well Shuttle™ Device
    Instructions of use for transfection of human umbilical vein endothelial cells (Lonza) with the P5 Primary Cell 96-well Nucleofector™ Kit
  • TechSheet - Aortic Endothelial Cell Systems
    Technical information sheet for Clonetics™ Aortic Endothelial Cells
  • Aortic Smooth Muscle Cells Induce Gene Expression in Aortic Endothelial Cells and Participate in In Vitro Angiogenesis
    Here, we show that the presence of aortic smooth muscle cells does have an impact on induction of genes expressed in aortic endothelial cells.
  • Instructions - Mammary Epithelial Cell System, HMEC
    Instructions for culturing Clonetics™ Mammary Epithelial Cells
  • TechSheet - Mammary Epithelial Cell System, HMEC
    Technical Information Sheet for CloneticsTM Mammary Epithelial Cell Systems
  • Instructions - Human Liver Endothelial Cells (HLEC)
    Instructions for proper use and culture of Human Liver Endothelial Cells
  • Transfection Protocol for HUVEC - Nucleofector™ I/II/2b Device Series
    Instructions of use for transfection of human umbilical vein endothelial cells with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S) using HUVEC Nucleofector™ Kit-OLD
  • Assessment of the Anti-angiogenic Effect of VEGFR2 siRNA in HUVEC
    Scientific poster for AACR 2015
  • Primary Human Umbilical Vein Endothelial Cells
    Tech note demonstrating the successful development of tube formation assay using primary HUVECs that can be used to visualize angiogenesis in real-time.
  • Primary Normal Human Cells and Media
    In Vivo Relevance. In Vitro Results. Updated and revised cell content.
  • Transfection Protocol for HMEC - 4D-Nucleofector™ Device
    Instructions of use for transfection of human mammary epithelial cells (Lonza) with the P3 Primary Cell 4D-Nucleofector™ X Kit
  • TechSheet - Sertoli Cell System, HSEC (MM-HSE-2305)
    Technical information sheet for Clonetics™Sertoli Cell System
  • Instructions - Sertoli Cell System, HSEC (MM-HSE-2305)
    Instructions for Clonetics™ Sertoli Cells
  • Transfection Protocol for HUVEC (Lonza) - Nucleofector™ I/II/2b Device Series
    Instructions of use for transfection of human umbilical vein endothelial cells (Lonza) with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)
  • First siRNA Library Screening in Hard-to-Transfect HUVEC and Jurkat Cells
    High throughput transfection of siRNA libraries has become a valuable tool in target identifi cation and validation. However, such screenings have so far been constrained to mostly easy-totransfect adherent cell lines. Lonza’s 96-well Shuttle® System, based on the well-established Amaxa® Nucleofector® Technology, extends these approaches to primary and diffi cult-to-transfect cells.
  • Nucleofector™ Technology – Guideline for Easy Set Up of RNAi Screening Experiments
    This guideline aims to help researchers in setting up a successful RNAiscreening experiment using the Nucleofector™ 96-well Shuttle™ System. The recommendations are based on our experience gained in using theNucleofector™ 96-well Shuttle™ System for screens with Thermo ScientificsiRNA libraries in difficult-to-transfect Jurkat T cells and primary HUVECcells2, combined with recommendations collected from the literature. It highlights important parameters that may influence the quality of cellbasedscreening results.
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