Nucleofector™ I/II/2b Software Updates
The software of a NucleofectorTM I, II or 2b Device comprises
- The operational software (firmware) which controls the system
- The NucleofectorTM Programs which correspond to the list of Optimized Protocols that are provided with the NucleofectorTM Kits which can be downloaded at www.lonza.com/protocols.
- The serial number of your NucleofectorTM Device which can be found on the type label on the bottom of your device
- The current software version installed (see below how to identify this)
NucleofectorTM I Device
NucleofectorTM II, IIS or 2b Devices
Nucleofector™ I Device
Nucleofector™ II Device
How To Identify the Software Version Currently Installed and Perform an Update
a) Viewing the software version currently installed
- For checking your current software version, switch on the device
- Find and note the second number presented on the display
Most recent program software version is R2.4 V2.5
b) Update Instructions
If your device has a program version lower than V2.5, please fill the form to order your Chip Card. Once you have received your new Chip Card, follow the instructions below:
- Turn on the NucleofectorTM Device
- Insert the Chip Card (chip forward and downward) into the small slot below the control panel within the grip of the NucleofectorTM I Device (see picture)
- To load the new programs press the “X” button
- A successful update is indicated as “C OK” shown on the display
- Remove the Chip Card from the NucleofectorTM Device
- Confirm by pressing the “X” button
- The display will first show either “R2.0”, “R2.1”, “R2.2”, “R2.3” or “R2.4” (depending on the age of the NucleofectorTM Device) and shortly afterwards V2.5
- The NucleofectorTM Device is now updated and ready for use
a) Viewing the software version currently installed
- To view the current firmware and program software version of your NucleofectorTM II or IIS, turn on the NucleofectorTM by using the power button.
- If the NucleofectorTM II/2b Device starts in the »Free Program Choice« mode, exit this mode by pressing the »X« button.
- Press the functional button »menu« several times until »Software Versions« appears.
- Note the complete version code.
The most recent versions are:
Nucleofector™ II Device
B2.81 R3.3
S3-8
* the program version can be updated via chip card
b) Update Instructions
If your device has a lower program version than the respective S*-* versions indicated on the table on the left for the respective model, please fill the form to order your Chip Card. Once received follow instructions below:
- Turn the device on (orange button).
- In order to upgrade the program list** in your NucleofectorTM II, IIS or 2b Device, insert the Chip Card (chip forward, facing down) into the Chip Card reader slot (see picture). Do not remove the Chip Card from the slot before the entire process is finished.
- After detecting the Chip Card, the display shows “Card found” and “Read Chip Card?”
- Press the “X” button to confirm.
- A running bar indicates the reading of the Chip Card. After doing so “Success” and “Pulstable 2 stored” is indicated. Press the “X” button.
- Now the display shows “Progr. found” and “Load programs?” Press the “X” button again.
- After a running bar has indicated the loading of the programs the display shows “OK” and “Remove card”.
- Remove card and press the “X” button twice.
- Press “menu” repeatedly until menu item “Software versions”. Now the last number displayed should the most recent for your variant as indicated in the table. The device is now updated.
** The stored custom programs will be neither deleted nor overwritten by upgrading novel non-custom optimized program.