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Pharma & Biotech

PASMC - Human Pulmonary Artery Smooth Muscle Cells

Catalog #: CC-2581

Cryopreserved ampule of Pulmonary Artery Smooth Muscle Cells (PASMC) containing ≥ 500,000 cells 

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Product Overview

Human Pulmonary Artery Smooth Muscle Cells (PASMC) are shipped in 3rd passage.

Human Pulmonary Artery Smooth Muscle Cells are guaranteed for 15 population doublings and to stain positive for alpha smooth muscle actin and negative for von Willebrand Factor VIII after differentiation.

All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.

Applications

  • Stroke
  • Diabetes
  • Vascular research  
  • Artherosclerosis
  • Hypertension

Content & Storage

Content

1 x Cryopreserved ampule PASMC containing ≥500,000 cells/vial

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

PASMC - Human Pulmonary Artery Smooth Muscle Cells

Human pulmonary artery smooth muscle cells(CC-2581) were stained with 1:1500 dilution of Anti Alpha-Smooth Muscle Actin dilution of (CBL171, Sigma). Immunofluorescence analysis was conducted using Secondary donkey polyclonal antibody Alexa Fluor Do anti Mo 594 Invitrogen (Thermo Fisher) A21203 at a 1:200 dilution. Nuclei were stained with Hoechst . In multi-well culture, PAMCSs appeared typically epitheloid (polygonal) forming monolayers of very low-level alpha-actin expressing cells. Rarely, a few intensely alpha-actin positive cells (arrows) were evident. These displayed distinct fusiform-elongated morphology.

PASMC - Human Pulmonary Artery Smooth Muscle Cells

Cells are isolated from human pulmonary artery and expanded prior to freezing (CC-2581). When plated & expanded, the cells express little to no alpha-actin unless first stimulated to differentiate.Cells were stained with Anti Alpha-Smooth Muscle Actin Chemicon (Millipore) CBL171 at 1:1500 dilution. Immunofluorescence analysis was conducted using Secondary donkey polyclonal antibody Alexa Fluor Do anti Mo 594 Invitrogen (Thermo Fisher) A21203 at 1:200 dilution. Nuclei were stained with Hoechst.

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