Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 25 million
Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 25 million
Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 25 million cells
Do you need a modified version of this cell product, such as different vialing sizes, matched immune cell isolations or isolations from special donors or species? Contact Lonza CellBio Services to discuss custom isolation, expansion, and testing options for your research
Product Overview
.Lonza is your ideal partner when you are looking to buy PBMC. Our Peripheral Blood Mononuclear Cells (PBMCs) are isolated from peripheral blood by apheresis and density gradient separation. Mononuclear cell (MNC) rich cells from leukapheresis are depleted of RBCs and platelets. MNC pools differ my donor and may contain, on average, 50% T cells, 20% monocytes, 10% B cells, 10% NK cells and may contain up to 5% granulocytes They are guaranteed to contain ≥25 million viable cells per ampoule. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. Cells are collected from healthy donors following IRB protocols A Certificate of Analysis is provided for each cell lot purchased.
Ability to Screen Different Donors
Sample representation in your experiment matters. We maintain an inventory of different donors in an effort to satisfy your sample representation requirements. You can choose a lot based on donor demographic characteristics, and screen multiple lots to find the lot(s) that work best for you. In addition, we can request repeat donors if you need additional cells from a particular donor.
Rich Source of Immune Cells
Peripheral blood mononuclear cells (PBMC) are a mixed population of single nucleus cells. They can be further purified to isolate individual cell types such as natural killer cells, T cells, and B cells. In addition, PMBCs are a rich source of monocytes, which can be directed to differentiate into either macrophages or dendritic cells through culture with various cytokines. Supported by an Optimized Media System LGM-3TM Lymphocyte Growth Medium was specifically designed for support and maintenance of lymphocytes and is optimized for serum-free culturing (additional cytokines may be necessary depending on application) Alternative media containing serum may also be acceptable.
Please contact Scientific Support for serum containing media recommendations. Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.
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Benefits
- Test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis (CoA) is provided for each cell lot purchased
Applications
- ELISPOT
- Proliferation assays
- Immunogenicity assays
- Cell therapy process development
- Humanized mice
- Biomarker discovery
Storage and Content
1 x Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 25 million cells
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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PBMC Based T Cell Proliferation Assay
Protocol instructions -
Instructions for Use - Dendritic Cell (DC) and T Cell Assay from Matched PBMCs
Protocol for DC and T Cell Assay from Matched PBMCs -
B-ALI Immune-Airway Co-Culture Model: Normal Human Bronchial Epithelial cells and Peripheral Blood Mononuclear Cells - Instructions for use
Protocol to culture B-ALI NHBE cells with PBMCs -
Procedure for thawing Poietics™ cells
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Tips For Optimizing Your CD8+ T Cell and Dendritic Cell Co-cultures
A co-culture between matured dendritic cells (DCs) and CD8+ T cells isolated from Lonza cryopreserved human peripheral blood mononuclear cells (PBMCs; part nos. CC-2702 – CC-2705) and we present three major takeaways from this process that are critical for successful co-culture and end-point analysis for these two cell types
Educational Material
Brochures, White Papers etc.
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Predicting Antibody Dependent Cell-Mediated Cytotoxicity (ADCC)
Genotypic and Phenotypic Screening of Human Peripheral Blood Mononuclear Cells -
Blood and Immune Cells
Fueling your next discovery with unmatched cell and donor variety -
An Innovative, Highly Efficient Method for Transfecting Large Quantities of Primary Human T-cells
A strong need exists for improved cancer treatment options. Lonza's approach relies on genetically modified, patient-derived cells like T-cells or natural killer cells which express chimeric antigen receptors (CAR) targeting cancer cells. -
A Non-Viral Method for Modifying and Expanding Primary T Cells
Learn how Lonza’s Cocoon® Platform, 4D-Nucleofector® LV Unit and X-VIVO® 15 Medium are integrated to enable non-viral genetic modification and subsequent expansion of primary T cells in a functionally-closed, automated workflow. -
Procedure for thawing Poietics™ cells
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Modeling Lung Inflammation in 3D
A Physiologically Relevant Model for Studying Interactions Between Immune Cells and the Lung Epithelium Using Lonza's Bronchial Air-Liquid Interface (B-ALI™) System -
Standardizing Donor Response Variation Using CEFT Peptide Stimulation Assays for the Detection of Reactive T Cells
We provide a protocol for a T cell stimulation assay using CEFT peptides that can be used to validate functional responses by the individual CD8+(cytotoxic) and CD4+(helper) T cell sub-populations for a given donor
