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Applications

Molecular Biology

Agarose for electrophoresis of nucleic acids

Lonza supplies a full range of gel electrophoresis products:

  • Molecular biology-grade agarose
  • A variety of specialty agaroses
  • Nucleic acid stains
  • DNA ladders and markers for multiple sizes
  • Loading buffers
  • Precast gels
  • Integrated gel electrophoresis systems

Choose the right combination of ingredients for your application. For example, the combination of specialty agarose Metaphor® and GelStar® Nucleic Acid Stain, will provide you with excellent resolution of size differences for small fragments after gel electrophoresis. Low-melt SeaPlaque® Agarose will help you recover your DNA or RNA with high integrity: the low melting point property of the agarose allows extraction of DNA or RNA from gels at a lower, non-denaturing temperature.

If you want to go fast and make no compromise on laboratory efficiency, the FlashGel® System works with precast and prestained gels for a fine resolution of DNA or RNA fragments within 2 to 7 minutes. The FlashGel® Recovery Cassette is a precast gel optimized to recover fragments for further downstream genetic engineering steps: the precast gels are nuclease-free and with the precast elution wells, you skip time-consuming gel extraction steps. If you prefer to work with your own gels, look out for the GTG® Symbol on the specialty agaroses. These ‘Genetic Technology Grade®’ agaroses have undergone additional quality control steps to guarantee no inhibition of downstream enzymatic reactions.

Browse through the many options and keep your goal in mind. Reach out to us in case you need support choosing the right combination for your specific gel electrophoresis application.

Agarose for electrophoresis of proteins

Using agarose for electrophoresis of proteins as an alternative approach to using polyacrylamide gels provides several benefits, including safety, efficiency and flexibility. Agarose gels can be run using vertical or horizontal systems and unlike polyacrylamide gels, agarose gels can be used effectively to separate proteins larger than 600,000 Da.

Covering a wide range of protein gel electrophoresis applications, our agaroses are optimized for the unique requirements of your molecular biology techniques. We provide specialty agaroses designed for:

  • electrophoresis of high-molecular-weight proteins (>600,000 Da),
  • electrophoresis of serum proteins / immunoelectrophoresis (IEP), and
  • isoelectric focusing (IEF).

Our agarose offerings also include agaroses appropriate for special applications, such as separation for efficient recovery of proteins, IEP of IgG and IgM, SPEP testing, crossed-immunoelectrophoresis and counter-immunoelectrophoresis (CIEP).

Custom molecular
biology services

When our standard products do not completely fit your needs, inquire about our custom capabilities to find an electrophoresis product that does. We have decades of experience providing agarose products and extensive knowledge of gel electrophoresis applications. As agarose specialists, we provide unique know-how and support on agarose gel electrophoresis applications. With our years of experience and technical knowledge, we can help you design the best solution for your agarose gel electrophoresis challenges. Collaborate directly with our team of scientists and project managers to understand feasibility, timelines and up-front costs. Our extended capabilities cover all product aspects, such as custom buffers, sizes, formats, concentrations, and well formats.

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Our agarose history

Lonza is one of the very few agarose suppliers in the world with experience in development, manufacture, quality control and distribution of agarose. Since the 1960s, our agarose products have been supplied under the names Cambrex, Biowhittaker®, FMC and Marine Colloids.

In 2007, with the acquisition of the agarose facility from Cambrex, Lonza became the leading innovator and a worldwide trusted supplier of agarose and precast gels. Since then, we have been marketing our agarose for electrophoresis product brands, including SeaKem®, NuSieve® and MetaPhor®, with Lonza’s brand promises of high quality and reliable performance.

With Lonza, you’re purchasing straight from the source, and straight from the agarose expert.