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Pharma & Biotech

SE Cell Line 384-well Nucleofector™ Kit

Catalog #: V5SC-1010
For high-throughput transfection of cell lines, e.g. Jurkat or MCF-7, when working with the 384-well HT NucleofectorTM System.

Product Overview

The SE Cell Line 384-well NucleofectorTM Kit is one of our three kits suited for high-throughput transfection of cell lines when working with the 384-well HT NucleofectorTM System.

This kit has been determined to be optimal for transfecting following cell types:

    • 3T3-L1, diff., 3T3-L1, undiff.
    • A-10, A7r5, 
    • BHK-21, BJ
    • C2C12, CaCo-2, Calu-3, Capan-1, Cos-7
    • DU145
    • GH3
    • HCT116, HeLa, HeLa S3
    • IMR-90
    • Jurkat (DSMZ), Jurkat E6.1
    • KG-1a, L6
    • MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MEG-01, MG-63, MRC-5
    • NCTC clone 929
    • Panc-1
    • T/G-HA VSMC, T-47D
    • U2OS, U-87 MG
    • WI-38

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 2 x 384 or 10 x 384 reactions.


  • High transfection efficiencies in difficult-to-transfect cell lines
  • Cells maintain functionality post transfection
  • Seamless integration into automated liquid handling environments
  • Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System


  • Transfection of low cell numbers down to 2 x 104 cell lines
  • High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries

Content & Storage


90 mL SE Cell Line NucleofectorTM Solution

20 mL Supplement 1

50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)

10 x 384-well NucleocuvetteTM Plate (20 μL)


Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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