Mouse Embryonic Fibroblast Nucleofector™ Kit 2
Mouse Embryonic Fibroblast Nucleofector™ Kit 2
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Product Overview
The NucleofectorTM 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. The Mouse Embryonic Fibroblast NucleofectorTM Kit 2 is one of our two kits suited for efficient transfectiion of primary mouse embyonic fibroblasts (MEF). Due to significant diversity between different MEF clones, we have developed two NucleofectorTM Kits (1 and 2) and an easy-to-use NucleofectorTM Starter Kit to determine which of the 2 kits is optimal for a specific MEF clone.
The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.
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Benefits
- High transfection efficiencies for primary cells
- Cells maintain functionality post transfection
Applications
- Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells -
- Low-throughput transfection in single cuvette format
Storage and Content
2.25 mL NucleofectorTM Solution for Mouse Embryonic Fibroblasts
0.5 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL)
25 Single use pipettes
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Transfection Protocol for MEF (Starter Protocol) - Nucleofector™ I/II/2b Device
Instructions of use for transfection of mouse embryonic fibroblasts (starter protocol) with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S) -
Transfection Protocol for Immortalized MEF - Nucleofector™ I/II/2b Device Series
Instructions of use for transfection of mouse embryonic fibroblasts with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)
