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Pharma & Biotech

Human Mesenchymal Stem Cell (hMSC) Osteogenic Differentiation Medium BulletKitTM

Catalog #: PT-3002

Culture system containing required basal medium and supplements for hMSC osteogenic differentiation

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Product Overview

Stem cells possess the unique ability to differentiate into multiple lineages of cells including osteoblasts. Lonza’s hMSC Osteogenic Differentiation BulletKitTM Medium is a serum containing medium designed to induce osteogenic differentiation of human bone marrow derived mesenchymal stem cells (hMSC) into mature, functionally active osteoblasts in as few as 14 days. Differentiation can then be validated by a variety of methods including the use of Lonza’s OsteoImageTM Bone Mineralization Reagent which provides a quantitative value related to the amount of osteogenic differentiation.

Partial List of Cells Differentiated with hMSC Osteogenic Differentiation BulletKitTM Medium*:

  • Human bone marrow derived stem cells
  • Human umbilical cord blood derived stem cells
  • Human umbilical cord matrix derived stem cells
  • C57BL/6 mice bone marrow derived stem cells
  • Human adipose derived stem cells
  • Human periodontal ligament derived stem cells
  • Human hamstring tendon derived stem cells

The hMSC Osteogenic Differentiation BulletKitTM Medium provides a differentiation medium guaranteed to induce osteogenic differentiation of bone marrow derived hMSCs into mature, functionally active osteoblasts; Each bottle of the hMSC Osteogenic Differentiation BulletKitTM Medium contains enough differentiation medium to fully differentiate up to 750,000 bone marrow derived hMSCs [approximately 25 wells in a 6-well plate format] into mature, functionally active osteoblasts.

The hMSC Osteogenic Differentiation Medium is offered as a BulletKitTM Medium (catalog no. PT-3002) which includes both the basal media and the necessary supplements for osteogenic differentiation of bone marrow derived hMSCs.

Lonza guarantees the performance of cells only if appropriate media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

*List of cells generated from customer data. Lonza only routinely uses this medium with Lonza’s human bone marrow derived mesenchymal stem cells and can therefore only guarantee the successful use of this media with these cells.

Content & Storage

Content

1 x hMSC Osteogenic Differentiation Basal Medium (PT-3924), 170 mL

1 x hMSC Osteogenic Differentiation SingleQuotsTM Supplements Kit (PT-4120)

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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