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EGMTM -2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM

Catalog #: CC-3202

Culture system containing EBMTM-2 Basal Medium (CC-3156) and EGMTM-2 MV Microvascular Endothelial Cell Growth Medium SingleQuotsTM supplements (CC-4147) required for growth of Microvascular Endothelial Cells

 

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Product Overview

Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used to grow CloneticsTM Primary Cells.    


The following Endothelial Cell Growth Medium BulletKitsTM are available:

  • EGMTM-2 BulletKitTM – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Contains 2% FBS and VEGF for rapid proliferation. Does not contain BBE.
  • EGMTM BulletKitTM – Optimized for HUVECs cat. no. CC-2517 and CC-2519, contains 2% FBS and BBE. Does not contain exogenous VEGF.
  • EGMTM-Plus BulletKitTM – Enhanced version of EGMTMTM Medium. Optimized for HUVECs cat. no. CC-2935. Contains 2% FBS and BBE. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGMTMTM Medium.
  • EGMTM-2MV BulletKitTM – Coronary and Iliac artery, human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. Does not contain BBE.

Lonza guarantees the performance of CloneticsTM/PoieticsTM Cells only if appropriate CloneticsTM/PoieticsTM Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

 

Content & Storage

Content

1 x EBMTM-2 Basal Medium (CC-3156),  500 mL

1 x EGMTM-2 MV SingleQuotsTM Supplement Pack (CC-4147) containing:

1 x Bottle FBS, 25.00 mL

1 x Natural Cap Vial with Hydrocortisone, 0.20 mL

1 x Gray Cap Vial with hFGF-B, 2.00 mL

1 x White Cap Vial with VEGF, 0.50 mL

1 x Yellow Cap Vial with R3-IGF-1, 0.50 mL

1 x Blue Cap Vial with Ascorbic Acid, 0.50 mL

1 x Green Cap Vial with hEGF, 0.50 mL

1 x Red Cap Vial with GA-1000, 0.50 m

 

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

All 3 tested lots of Human Lung Microvascular Endothelial Cells (HMVEC-L, cat no. CC-2527) show better morphology and confluency when grown in EGM™ 2 MV compared to EGM™ MV

Cells were planted in a T25 flask and cultured for up to 9 days before Passage 1. Then, cells were split at 1 to 3 dilution and cultured until they reached 100% confluence. The cells need to reach 90-100% confluency in 1st passage and 100% confluency in the 2nd passage by day 9 to pass confluency test.

All 3 tested lots of Human Lung Microvascular Endothelial Cells (HMVEC-L, cat no. CC-2527) show better morphology and confluency when grown in EGM™ 2 MV compared to EGM™ MV

To evaluate morphology, photomicrographs are taken at 20X with a Leica Dmi1 light microscope, when the cells reach 90-100% confluence (P1) and 100% confluence for P2. Passing morphology includes cells that have 1) tightly packed or «cobblestone» appearance and 2) bright and smooth cell borders.