Trypan Blue, 0.4% Solution

Product Overview

Trypan Blue Stain is a stain used to distinguish viable from nonviable cells. Nonviable cells will absorb the dye and appear blue, while viable cells will exclude the dye.

Cells should be in a single cell suspension in buffered saline before counting.

Since trypan blue has a higher affinity for serum protein than for cellular proteins, suspending the cells in medium containing serum will generate a dark background.

Commonly used to assess cell viability.