text.skipToContent text.skipToNavigation
Need help? Please contact us

Stellate cells, human cryopreserved, passage 1

Stellate cells, human cryopreserved, passage 1

Catalog #: HUCLS

Human Cryopreserved Stellate cells derived from liver

 

Note:  This product to be discontinued following depletion of current inventory.  Please use our 200K and 1M yield parts for future needs (HUCLS-200K, HUCLS-1M)

 

 

Compare

Product Overview

 

Stellate cells are a resident cell type of the liver primarily functioning to store retinoids. In response to liver damage, stellate cells rapidly lose the stored retinoids and differentiate into a proliferating fibroblast-like cell that begins depositing collagen matrix. This activity causes buildup of collagen in the liver eventually leading to cirrhosis.

Stellate cells can be isolated from liver tissue, enriched, and placed into cell culture. When placed into culture, stellate cells begin to immediately differentiate into the activated state.

Human Cryopreserved Stellate Cells, 100K cells/vial,  are stellate cells enriched from human liver isolations and grown to >80% confluency and then cryopreserved.

Human Stellate Growth Medium (Catalog MCST250) is available to support optimal culture health and growth characteristics of stellate cells.

Characterization performed:

  • Post-thaw plating to observe characteristic stellate morphology. 
  • Flow cytometry using antibodies for Vinculin, Vimentin, and α-SMA

 

Request More Info

Benefits

  • Low Passage
  • High activation potential

Applications

  • ADMETox
  • Liver Disease
  • NASH

Storage and Content

Cryo Vial

100,000 cells/ vial

Return to Top

SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • The Importance of Cell-subtype Selection in Creation of a 3D Liver Fibrosis Model

    Data sheet detailing 3D liver fibrosis model in collaboration with Visikol
  • Instructions - Cryopreserved Human Stellate Cells

    Technical Instructions for culturing human stellate cells
Return to Top

Educational Material

Brochures, White Papers etc.

  • Webinar - Hepatocytes in the Drug Development Pipeline

    Overview of how primary hepatocytes are used in drug development
  • Supporting In Vitro ADMETox with an Advanced Portfolio of Primary Cells and Media

    Reference to Help Get Started on Primary Cell Culture for ADMETox Applications
Return to Top
Data

The Importance of Cell-subtype Selection in Creation of a 3D Liver Fibrosis Model

Data sheet detailing 3D liver fibrosis model in collaboration with Visikol

Return to Top

Subscribe to our eNewsletter

Keep up to speed on the latest scientific developments, events, tips and tools from Lonza.

Interested in our webinars?

Need a break from your daily routine? Grab a coffee and check out our free webinars. 

Build In Vivo-Relevant Liver Models by Adding Non-Parenchymal Cells

Hepatocyte Applications & Product Info

Learn how to create more complex liver models with hepatic non-parenchymal cells, including Kupffer cells, stellate cells, and liver sinusoidal endothelial cells.