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4D-Nucleofector® Core UnitCatalog #: AAF-1003B
The controlling unit of the 4D-Nucleofector® System is suited to handle various functional units for efficient transfection of hard-to-transfect cell lines and primary cells in different formats.
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4D-Nucleofector® Y UnitCatalog #: AAF-1003Y
Enables the direct Nucleofection® of cells in adherence in 24-well culture plates
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Primary Cell Optimization 4D-Nucleofector® Y KitCatalog #: V4YP-9A48
For rapid determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® Y Unit.
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AD2 4D-Nucleofector® Y KitCatalog #: V4YP-2A24
For adherent electroporation of primary cells, e.g. fibroblasts, when working with the 4D-Nucleofector® Y Unit.
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AD1 4D-Nucleofector® Y KitCatalog #: V4YP-1A24
For adherent electroporation of primary cells, e.g. neuronal or endothelial cells, when working with the 4D-Nucleofector® Y Unit.
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Cell Line Optimization 4D-Nucleofector® X KitCatalog #: V4XC-9064
For rapid determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector® X Unit.
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P4 Primary Cell 4D-Nucleofector® X Kit LCatalog #: V4XP-4024
For efficient transfection of specific primary cells, e.g. mouse B-cells or mouse immature DCs, in the 4D-Nucleofector® X Unit (100 µL format).
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P5 Primary Cell 4D-Nucleofector® X Kit SCatalog #: V4XP-5032
For efficient transfection of specific primary cells, e.g. HUVEC, in the 4D-Nucleofector® X Unit (20 µL format).
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P2 Primary Cell 4D-Nucleofector® X Kit SCatalog #: V4XP-2032
For efficient transfection of specific primary cells, e.g. mouse macrophages and NHDF, in the 4D-Nucleofector® X Unit (20 µL format).
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Primary Cell Optimization 4D-Nucleofector® X KitCatalog #: V4XP-9096
For rapid determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® X Unit.
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4D-Nucleofector® ManualManual for second generation 4D-Nucleofector Core, X, Y, 96-well, and LV Unit PRO
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Webinar – Efficient Transfection of Biologically Relevant Cells in Immunology Research (pdf)Lonza_Webinars_Webinar__Efficient_Transfection_of_Biologically_Relevant_Cells_in_Immunology_Research.pdf
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Nucleofector Devices - Guarantee Sales SheetNew version reflecting the revised concept.
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4D-Nucleofector® Manual – Second Generation with LV UnitManual for next generation 4D-Nucleofector® Core, X, Y, 96-well, and first-gen LV Unit
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How it Works - 4D-Nucleofector® Y Unit (Video Tutorial)Workflow overview for using the 4D-Nucleofector™ Y Unit -
References - X-Vivo™Overview of relevant references
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Generation of hiPSCs from Cord Blood Cells With the 4D-Nucleofector® SystemA scientific paper by Lonza, outlining the generation of iPSCs from Cord Blood Cells using the 4D-Nucleofector® System
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A Novel, Chemically Defined, Serum-Free Cell Culture Medium for Expansion of Transfected T CellsIn this study, we present data generated with TheraPEAK® T-VIVO®, a novel, chemically defined, non-animal origin cell culture medium utilized to grow T cells in the absence of serum, before and after transfection with the Nucleofector® Technology.
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Next Generation 4D-Nucleofector® System Toss SheetSee at-a-glance product characteristics of the next generation 4D-Nucleofector System, including Core Unit, X Unit, Y Unit, LV Unit and 96-well Unit descriptions, data about transferability of Nucleofection conditions and ordering information.
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Basic Transfection Protocol for Neural Cells (Adher.) - 4D-Nucleofector™ DeviceInstructions of use for transfection of adherent primary mammalian neurons with the AD1 Primary Cell 4D-Nucleofector™ Y Kit