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MEGM™ Mammary Epithelial Cell Growth Medium BulletKit™

MEGM™ Mammary Epithelial Cell Growth Medium BulletKit™

Catalog #: CC-3150

Culture system containing MEBMTM Basal Medium (CC-3151) and MEGMTM SingleQuotsTM Supplements (CC-4136) required for growth of Mammary Epithelial Cells.

 

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Product Overview

MEGMTMBulletkitTM, is also sold as separate basal medium and supplements.

MEGMTMTM Mammary Epithelial Cell Growth Medium has been optimized for the growth of Mammary Epithelial cells in a serum-free environment. This media system has been referenced in over 130 unique publications primarily focusing on breast cancer research. 

Did you know our customers have used MEGMTMTM BulletKitTM not only for Lonza's primary HMECs but also to culture well-established normal breast cell lines such as MCF-10A and MCF-12A? 

MEGMTMTM BulletKitTM is optimized and readily available media kit giving researchers the confidence and the convenience of a system that works to support their cells. This is especially important for researchers who are utilizing normal breast cell lines as a stepping stone to primary HMEC cell culture. Working with the same media between cell lines and primary cells can reduce variability within the experiments and simplifies the shift for researchers. In addition, if primary HMECs are utilized as a side-by-side control with normal breast cell lines, the MEGMTMTM BulletKitTM media may also offer cost-benefits since customers can use the same media to support both cell types.

Purchase our Mammary Epithelial Cell Culture Kit (catalog no. CC-2551B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of mammary epithelial cells (catalog no. CC-2551) and MEGMTMTM BulletKitTM Growth Media (catalog no. CC-3150)

 

 

  

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Storage and Content

1 x MEBMTM Basal Medium (CC-3151), 500 mL

1 x MEGMTM SingleQuotsTM Supplement Pack (CC-4136) containing:

1 x Orange Cap Vial with BPE, 2.00 mL

1 x Green Cap Vial with hEGF, 0.50 mL

1 x Lilac Cap Vial with Insulin, 0.50 mL

1 x Natural Cap Vial with Hydrocortisone, 0.50 mL

1 x Red Cap Vial with GA-1000, 0.50 mL

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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • Primary Cells and Media Reference Guide

    A comprehensive collection of Lonza primary cell and media products supporting many research areas.
  • Instructions - Mammary Epithelial Cell System, HMEC

    Instructions for culturing Clonetics™ Mammary Epithelial Cells
  • TechSheet - Mammary Epithelial Cell System, HMEC

    Technical Information Sheet for CloneticsTM Mammary Epithelial Cell Systems
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Educational Material

Brochures, White Papers etc.

  • Lonza's Bulletkit™ for Primary Cell Growth

    Primary Cells are Different from Cell Lines. Why use the Same Classical Media? Learn how Lonza primary cells and media can support your cell research
  • Primary Cells and Media Reference Guide

    A comprehensive collection of Lonza primary cell and media products supporting many research areas.
  • Epithelial Cells Concentration Guide

    Epithelial cell culture media guide with SingleQuots® concentration.
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Selected publications out of many where our customers have used MEGM™ Growth Media with various normal breast cells.

  • Savanur, et al. shows Lonza's HMECs were cultured in Lonza's MEGM™ Media alongside breast cancer cell lines in the respective media to understand the inhibitory effects of Lectin on proliferation of breast cancer cells
  • Park, et. al. PLoS ONE 10(6) demonstrates how MCF-10A and MCF-12A were cultured in MEGM™™ alongside breast cancer cell lines in their respective media to examine the effects of cold atmospheric plasma on epigenetic alterations
  • Kmiecik, et al. 2015. PLoS ONE 10(5) shows MEGM™ Medium's compatibility with immortalized hTERT-HME1 cells in a study of MT3-overexpression on proliferation, invasiveness, migration,and tumorigenesis of breast cancer cells

Data

Lonza’s HMEC cells cultured in MEGM™ BulletKit™ Medium vs. other commercially available systems

Graph showing the propagation potential of Lonza’s HMEC cells cultured in MEGM™ BulletKit™ Medium vs. other commercially available systems

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