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SF Cell Line 4D-Nucleofector™ X Kit L

SF Cell Line 4D-Nucleofector™ X Kit L

Catalog #: V4XC-2012
For efficient transfection of difficult-to-transfect cell lines, e.g. K-562, Neuro-2A or RAW264.7, in the 4D-NucleofectorTM X Unit (100 µL format).
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Product Overview

The SF Cell Line 4D-NucleofectorTM X Kit L is one of our three 4D-NucleofectorTM Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL NucleocuvetteTM Vessel format when working with the 4D-NucleofectorTM X Unit.

This kit has been determined to be optimal for transfecting following cell types:

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 12 or 24 reactions.

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Benefits

  • High transfection efficiencies in difficult-to-transfect cell lines

Applications

  • Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells
  • Low throughput Applications s

Storage and Content

2 x 0.675 mL SF Cell Line NucleofectorTM Solution
2 x 0.15 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
12 x 100 µL NucleocuvetteTM Vessel
12 x Single use pipettes

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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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Educational Material

Videos

  • How it Works - 4D-Nucleofector® System (Video Tutorial)

    Workflow overview for using the 4D-Nucleofector™ System
  • How it Works - 4D-Nucleofector™ X Unit (Video Tutorial)

    Workflow overview for using the 4D-Nucleofector™ X Unit
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