EGM®-2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKit®
EGM®-2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKit®
Culture system containing EBM®-2 Basal Medium (CC-3156) and EGM®-2 MV Microvascular Endothelial Cell Growth Medium SingleQuots® supplements (CC-4147) required for growth of Microvascular Endothelial Cells
Do you need a quote right away? Add this product to your shopping cart, go to your cart and click on “Generate Quote”. Your quote will be available straight away to view, save online, and download in PDF format.
Product Overview
Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used to grow Clonetics® Primary Cells.
The following Endothelial Cell Growth Medium BulletKits® are available:
- EGM®-2 BulletKit® – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Final concentration of FBS in the mixed BulletKit is 2%. Contains VEGF for rapid proliferation. Does not contain BBE.
- EGM® BulletKit® – Optimized for HUVECs cat. no. CC-2517 and CC-2519. Contains BBE. Final concentration of FBS in the mixed BulletKit is 2%. Does not contain exogenous VEGF.
- EGM®-Plus BulletKit® – Enhanced version of EGM® Medium. Optimized for HUVECs cat. no. CC-2935. Contains BBE. Final concentration of FBS in the mixed BulletKit is 2%. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGM® Medium.
- EGM®-2MV BulletKit® – Coronary and Iliac artery, human microvascular endothelial cells. Final concentration of FBS in the mixed BulletKit is 5%. Contains VEGF for rapid proliferation. Does not contain BBE.
Lonza guarantees the performance of Clonetics®/Poietics® Cells only if appropriate Clonetics®/Poietics® Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.
If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.
Request More InfoStorage and Content
1 x EBM®-2 Basal Medium (CC-3156), 500 mL
1 x EGM®-2 MV SingleQuots® Supplement Pack (CC-4147) containing:
1 x Bottle FBS, 25.00 mL
1 x Natural Cap Vial with Hydrocortisone, 0.20 mL
1 x Gray Cap Vial with hFGF-B, 2.00 mL
1 x White Cap Vial with VEGF, 0.50 mL
1 x Yellow Cap Vial with R3-IGF-1, 0.50 mL
1 x Blue Cap Vial with Ascorbic Acid, 0.50 mL
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 m
Download our Endothelial Cell Growth Medium Concentration Guide
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
-
TechSheet - Keratinocyte Cell Systems
Technical information sheet for Clonetics® for Keratinocyte Cell Systems -
TechSheet – Endothelial Cell System Medium Products
Technical information sheet for Clonetics® Endothelial Cell Medium Products -
Primary Cells and Media Reference Guide
A comprehensive collection of Lonza primary cell and media products supporting many research areas. -
Instructions & Technical Info - Endothelial Cell Systems
Technical Information and Instructions for Proper Use of Endothelial Cells -
TechSheet, Instructions - Immo. HMVEC-d
Technical information and instructions for culturing Clonetics® Immortalized Human Dermal Blood Lymphatic Microvascular Endothelial Cells
Educational Material
Brochures, White Papers etc.
-
Lonza's Bulletkit® for Primary Cell Growth
Primary Cells are Different from Cell Lines. Why use the Same Classical Media? Learn how Lonza primary cells and media can support your cell research -
Primary Cells and Media Reference Guide
A comprehensive collection of Lonza primary cell and media products supporting many research areas.
All 3 tested lots of Human Lung Microvascular Endothelial Cells (HMVEC-L, cat no. CC-2527) show better morphology and confluency when grown in EGM® 2 MV compared to EGM0« MV
Cells were planted in a T25 flask and cultured for up to 9 days before Passage 1. Then, cells were split at 1 to 3 dilution and cultured until they reached 100% confluence. The cells need to reach 90-100% confluency in 1st passage and 100% confluency in the 2nd passage by day 9 to pass confluency test.
All 3 tested lots of Human Lung Microvascular Endothelial Cells (HMVEC-L, cat no. CC-2527) show better morphology and confluency when grown in EGM® 2 MV compared to EGM® MV
To evaluate morphology, photomicrographs are taken at 20X with a Leica Dmi1 light microscope, when the cells reach 90-100% confluence (P1) and 100% confluence for P2. Passing morphology includes cells that have 1) tightly packed or «cobblestone» appearance and 2) bright and smooth cell borders.
