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What is a microplate reader in endotoxin testing?

A microplate reader is an instrument for endotoxin testing that generates the raw measurement data required for compliant analysis and reporting. The instrument performs kinetic or endpoint measurements to detect and quantify endotoxins accurately. WinKQCL® Endotoxin Detection and Analysis Software then analyzes and reports the data, creating a complete and compliant endotoxin testing workflow.

Why choose Lonza microplate readers for endotoxin testing?

Lonza microplate readers are purpose-built endotoxin testing equipment for bacterial endotoxin testing. These 96-well plate readers are designed to deliver precise, consistent results and work with WinKQCL® Software and Lonza reagents (PYROGENT® 5000, Kinetic-QCL®, PyroGene® rFC Assays).

Key advantages for QC directors and lab managers:

  • Ecosystem optimization: Designed to work with WinKQCL® Software and Lonza assays for high performance and low risk.
  • Flexibility: Choose absorbance-only, fluorescence-only, or multimode (absorbance + fluorescence) endotoxin testing equipment based on your assay requirements.

Nebula® Absorbance Reader

The Nebula® Absorbance Reader integrates with the latest WinKQCL® Software to support data integrity compliance, streamline training, and reduce validation burden.

PyroWave® XM Fluorescence Reader

The PyroWave® XM Fluorescence Reader is part of a quantitative endotoxin detection system that supports the PyroGene® rFC Endpoint Fluorescent Assay.

Nebula® Multimode Reader

Conduct traditional kinetic LAL assays and recombinant Factor C technology testing with one easy-to-use instrument for endotoxin testing.

Which microplate reader is best for endotoxin testing? 

The best instrument for endotoxin testing depends on the assay type (LAL or rFC) and the measurement capabilities required, as shown below.

Nebula® Absorbance Reader PyroWave® XM Fluorescence Reader Nebula® Multimode Reader
Kinetic Turbidimetric LAL Assay + - +
Kinetic Chromogenic LAL Assay + - +
Recombinant Factor C (rFC) Assay - + +
WinKQCL® integration + + +
Incubator option + + +
Shaker option (Shaking) + + +
Wavelength range (nm) 230 – 1,000 nm 200 – 850 nm** 230 – 1000nm (Absorbance), 230 – 850nm (Fluorescence)
Fluorescence sensitivity n/a Fluorescein @ 1pM/well in a 96 well plate Fluorescein, < 20 μM Top read, 100 μM Bottom read
Optical setup (Wavelength selection) Monochromator-based, precise selection of wavelength possible Filter-based with easy-to-swap filter cube with 2 filter configurations Monochromator-based, precise selection of wavelength possible
Filters supplied n/a - Excitation filter 380/20 nm;
- Excitation filter 485/20 nm;		 n/a

**Additional filters must be purchased for additional wavelengths and read capabilities

Questions? We've got answers

    

What should laboratories consider when selecting a microplate reader for endotoxin testing?

Laboratories should consider measurement capability, temperature control, data reliability, and compatibility with analysis software. Selecting an instrument for endotoxin testing that aligns with validated workflows helps ensure consistent results and simplifies implementation in regulated environments.
    

Do Lonza readers support rFC assays?

Yes. Both Nebula® Multimode and PyroWave® XM Readers support the PyroGene® rFC Endpoint Fluorescent Assay.
        

How can laboratories reduce variability in endotoxin testing results?

Laboratories can reduce variability by using compatible endotoxin testing equipment and software, standardizing workflows, and minimizing manual data handling. Consistent measurement and analysis approaches help produce reliable endotoxin results over time.
        
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