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Cell isolations from various tissues

Our staff  with over 30 years of custom isolation experience is here to assist in determine the optimal method to isolate your desired cell type. With the capability to perform enzymatic digestions, mechanical separation, density gradient centrifugation, immunomagnetic separation, and selective culturing, we are ready to find the ideal isolation method to yield optimal results. Isolations can be further optimized by utilizing Lonza’s extensive range of cell culture media options. Final products can be offered fresh (plated or suspension), cryopreserved (cell suspension or pellet), or further utilized for RNA/DNA services to suit your needs.

We have successfully isolated many unique cell types, including, but not limited to:

  • Canine & Porcine Kidney Cells
  • Cynomolgus monkey lung microvascular endothelial cells
  • Feline and Porcine renal cells
  • Human Adult Foreskin Fibroblasts
  • Human renal pericytes
  • Human renal podocytes
  • Human retinal pericytes
  • Human Valvular Endothelial Cells (VEC) and Valvular Interstitial Cells (VIC)
  • Matched Canine Mesenteric Artery, Portal Vein, and Hepatic Artery Endothelial Cells
  • Matched Gamma Delta T-Cells & CD3+ Pan T Cells
  • Matched Peritoneal, Pericardial, & Pleural Mesothelial Cells
  • Pooled Bone Marrow MNCs
  • Pooled Cord Blood MNCs
  • Splenocytes/Spleen Derived Monocytes


To request further information or quotes, please get in touch with us.

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Modifications to existing catalog products

CellBio Services provides the option to manipulate our standard catalog products to suit your experimental needs.

Examples include:

  • Additional donor characteristics
  • Donor-matched cell sets
  • Pooled donors
  • Cell expansions
  • Additional testing
 


To request further information or quotes, please get in touch with us.

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Limited edition products through CellBio Services

Lonza CellBio Services occasionally obtains excess or additional cells from custom isolations that can be offered as individual cryopreserved vials.

These Limited Edition Cells are available in limited inventory and may not be available again after inventory is depleted.

Current stock of Lonza CellBio Services Limited Cells includes the following cell types:
     

Product description Catalog #
Human Dermal Fibroblasts; lesion (psoriasis)          
       		 219736
Human Epidermal Keratinocytes; non-lesion (psoriasis)  219735
Human Peripheral Blood Mononuclear Cells (psoriasis)   219738
Adult Intestinal MyoFibroblasts (InMyoFib) 28177B
IPF Bronchial Epithelial Cells (DHBE-IPF) 24586B
IPF Small Airway Epithelial Cells (SAEC-IPF) 24586C
                          

For more information on our limited edition inventory of primary cells, please get in touch with us.

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P-HDF - Diseased Human Dermal Fibroblast (Lesion)

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P-HEK - Diseased Human Epidermal Keratinocytes (Non-Lesion)

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D-HPBMC - Diseased Human Peripherial Blood Mononuclear Cells (Psoriasis)

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Primary cell and cell line expansion services

Use Lonza as an extension of your cell culture facility. Lonza's CellBio Services team can expand your primary cell or cell line according to specific standards and cryopreserve the cells in your preferred format. This will allow you to focus on your assays and results. 
We can provide:

  • Expansions of adherent cell types in batches up to 2 x 1010 cells, or suspension cell types in batches up 5 x 1010 cells
  • Batch sizes up to 1500 vials
  • Production capabilities in a variety of formats including CellStacks, culture flasks, closed system and suspension cultures (up to 12L)
  • Generation of master and working cell banks
  • Batch Testing and CoA generation

To request further information or quotes, please get in touch with us.

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Custom research use cell culture media


When standard catalog media offerings do not quite meet your research needs, CellBio Services provides the option to manipulate our standard catalog media products to suit your experimental research needs. Alternatively, CellBio Services can generate completely new media based on your supplied formulation. Our production facilities can accommodate custom research use only grade liquid formulation productions from 500 mL up to 10,000 L. If you prefer powder, our production facilities can accommodate custom research grade powder formulation productions from 1 kg to 7,000 kg. Examples include:

  • Lonza primary and stem cell growth and differentiation media with added/removed components and/or in custom fill volumes or vessels
  • Lonza Classical/Specialty media with added/removed components (i.e. SILAC or phenol red free media)
  • Custom packaging (Vials, Bottles, Bags, Drums, etc)
  • Custom fill sizes
  • Custom cell culture and testing services

To request further information or quotes, please get in touch with us.

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Pancreatic islets

Some of the major challenges for researchers are the acquisition and availability of high-quality islets. Lonza‘s Fresh Human Pancreatic Islets (Islets of Langerhans) provide researchers with easy access to islets to support their research.

Pancreatic islets of Langerhans

Product overview:

  • Enhanced packaging – Orders ship in Wilson Wolf flasks for optimal viability
  • Guaranteed quality – Tested for quantity, purity, viability and bacterial sterility
  • Flexible – Request cells in various product sizes; no forecasts needed
  • Specialized support – Technical team dedicated to support product inquiries
  • Customize your order – Additional quality testing and more options are available upon request

Some of the major challenges for researchers are the acquisition and availability of high-quality islets. Lonza‘s Fresh Human Pancreatic Islets (Islets of Langerhans) provide researchers with easy access to islets to support their research. These islets are made-to-order. Islets are supplied as IEQ (islet equivalents) and shipped in a Wilson Wolf gas permeable flask to maintain optimal viability. Each batch is isolated from normal human donors and tested negative for HIV-1, HBV and HCV. Each batch is tested for quantity, viability, purity and bacterial sterility. The product is guaranteed to meet minimum specifications indicated by the certificate of analysis. Pancreatic islet culture medium is available for purchase with each shipment.

Pancreatic islets can be offered to customers as pancreatic tissue becomes available. If special donor characteristics are requested, the order will be subject to donor availability and may increase lead time. Sterility and any additional QC results requested will be available post-islet shipment. 

How to order

Contact your Lonza Representative or CellBio Services to receive the Pancreatic Islet request form. Completed request forms will be routed to our CellBio Services Team, who will contact you shortly after the form is submitted to provide details on availability and pricing. Customer Service will contact you to confirm your order when it is placed. Courier tracking information for your shipment will be sent to the email address provided in the order form once the product has been shipped.

Related Tools: Cells & Transfection Database

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Pancreatic Islets

Pancreatic islet cells stained with dithizone (DTZ)

Instructions – Pancreatic Islets

Clonetics Fresh Human Pancreatic GSIR DNA Quantitation Oxygen Consumption Rate OCR Beta Cell Composition. Download Document

Terms and Conditions

Download Document

Lonza proliferating cells

Viable cells, plated & ready to use


In addition to cryopreserved cell ampules Lonza also offers plated cells. Lonza Proliferating Cells are available in a variety of convenient flask and plate formats.

Proliferating cells are delivered within 5 to 7 days after plating depending on confluence. All cells are shipped subconfluent, however confluency at the time of shipment varies by cell type. A new Cryovial is used for each new batch using only Lonza Media and Reagents. This procedure ensures viable cells of optimal quality.

Benefits of Lonza proliferating cells

  • Save valuable time and materials by using viable cells: No need for expensive thawing and culture expansion. Just order the number of vessels you need.
  • Proof your results: Validate your cell line experiments with a reliable ready to use primary cell culture

Characteristics of Lonza proliferating cells

  • More than 100 cell types available – normal and diseased
  • Variety of flasks and plates
  • Ready-to-assay in plate formats
  • Option to select which batch of cryopreserved cells to use
  • 40+ years of experience in primary cells with most publications

Proliferating cells are made-to-order. Typical lead times can be between 2-4 weeks depending upon when the order is placed. Please contact CellBio Services to get additional information on delivery times for your specific product of interest. We advise that you order an appropriate Lonza Media Bulletkit® and a Reagent Pack (cat. # CC-5034) with the cells to ensure best performance.

To request further information or quotes, please get in touch with us.

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