SF Cell Line 4D-Nucleofector® X Kit S
SF Cell Line 4D-Nucleofector® X Kit S
For efficient transfection of difficult-to-transfect cell lines, e.g. K-562, Neuro-2A or RAW264.7, in the 4D-Nucleofector® X Unit (20 µL format).
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Product Overview
The SF Cell Line 4D-Nucleofector® X Kit S is one of our three 4D-Nucleofector® Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette® Strip format when working with the 4D-Nucleofector® X Unit.
This kit has been determined to be optimal for transfecting following cell types:
- 293-F (FreeStyle), 32D
- A-20, A2058, A375, A431, A549, AGS, ARPE-19
- BetaTC/6, BxPC-3
- C6, CCRF-CEM, CHO-K1, CHO-S (Invitrogen), Cos-1
- EL4
- FDC-P1
- H9c2 (2-1), HEK293, Hel9217, HepG2, High 5, HL-60, HMC-1-8, HT-1080, HT-29, Hut-78, HUV-EC-C
- IMR-32
- J774A.1
- K-562
- KG-1
- L-428, LNCaP
- MDA-MB-453, MDBK, Molt-4, MV-4-11
- Nalm-6, NB-4, NCI-H1299 [H1299], Neuro-2a [N2a], NG108-15, NS0
- P19, P815, PC-12, PC-3
- Raw 264.7, RBL-1, RBL-2H3, Reh, Rin m5F, RPMI 8226
- S2, S49, SaOS, SF9, SH-SY5Y, SK-BR-3, SK-N-AS, SK-N-MC, SK-N-SH, SK-OV-3, Sp2/0-Ag14, SW480
- T2, T84, TF-1
- U266B1, U-937
- Vero
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
Request More InfoBenefits
- High transfection efficiencies in difficult-to-transfect cell lines
- Cells maintain functionality post transfection
- Conditions are transferable between 4D-Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System
- Transferability to 4D-Nucleofector® LV Unit
Applications
- Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells
- Medium throughput applications
- Optimization of transfection conditions e.g. substrate titrations
Storage and Content
1 x 0.675 mL SF Cell Line Nucleofector® Solution
1 x 0.15 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 16-well Nucleocuvette® Strips (20 μL)
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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4D-Nucleofector® Protocol for TF-1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for TF-1 -
4D-Nucleofector® Protocol for A-375
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for A-375 -
4D-Nucleofector® Protocol for CCRF-CEM
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for CCRF-CEM -
4D-Nucleofector® Protocol for C6 Glioma
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for C6 Glioma -
4D-Nucleofector® Protocol for L428
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for L428 -
4D-Nucleofector® Protocol for FDC-P1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for FDC-P1 -
4D-Nucleofector® Protocol for CHO-K1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for CHO-K1 -
4D-Nucleofector® Protocol for EL4
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for EL4 -
4D-Nucleofector® Protocol for K562
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for K562 -
4D-Nucleofector® Protocol for HL-60
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for HL-60
