Human T Cell Nucleofector® Kit
Human T Cell Nucleofector® Kit
For efficient transfection of primary human T cells in the Nucleofector® I/II/2b System
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Product Overview
The Nucleofector® 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector® Kit is the optimal kit for efficient transfection of primary human T cells (unstimulated or pre-stimulated).
The cell type-specific Optimized Protocol is available in our knowledge database. In this Optimized Protocols the best Nucleofection Conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
Request More InfoBenefits
- High transfection efficiencies for primary cells
- Cells maintain functionality post transfection
Applications
- Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells
- Low-throughput transfection in single cuvette format
Storage and Content
2.25 mL Nucleofector® Solution for Human T Cells
0.5 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL), 25 Single use pipettes
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
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