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P4 Primary Cell 4D-Nucleofector® X Kit L
Catalog #: V4XP-4012
For efficient transfection of specific primary cells, e.g. mouse B-cells or mouse immature DCs, in the 4D-Nucleofector® X Unit (100 µL format).
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TheraPEAK® 4D-Nucleofector® LV Reservoirs, weldable (5 pcs)
Catalog #: G4LR-1501W
For use in combination with TheraPEAK® Nucleocuvette® Cartridges, supporting the use of the 4D-Nucleofector® LV Unit in a GMP environment.
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SF Cell Line 4D-Nucleofector® LV Kit L
Catalog #: V4LC-2002
For larger scale transfection of cell lines, e.g. suspension CHO or HEK293, in the 1 mL NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit
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P3 Primary Cell 4D-Nucleofector® LV Kit L
Catalog #: V4LP-3002
For larger scale transfection of primary cells, e.g. human T cells or CD34+ hematopoietic stem cells in the 1 mL NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit
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Primary Cell Optimization 96-well Nucleofector® Kit
Catalog #: V4SP-9096
For rapid determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® 96-well Unit.
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TheraPEAK® LV Nucleocuvette® Cartridge, weldable (5 pcs)
Catalog #: G4LN-7520W
For use in combination with TheraPEAK® Nucleofector® Solution Sets, supporting the use of the 4D-Nucleofector® LV Unit in a GMP environment.
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SF Cell Line 4D-Nucleofector® LV Kit XL
Catalog #: V4LC-2020
For larger scale transfection of cell lines, e.g. suspension CHO or HEK293, in the LV NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit
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SF Cell Line 4D-Nucleofector® LV Kit XL (5 x 1 RCT)
Catalog #: V4LC-2520
For larger scale transfection of cell lines, e.g. suspension CHO or HEK293, in the LV Nucleocuvette® Cartridge format when working with the 4D-Nucleofector® LV Unit
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P3 Primary Cell 4D-Nucleofector® LV Kit XL
Catalog #: V4LP-3020
For larger scale transfection of primary cells, e.g. human T cells or CD34+ hematopoietic stem cells in the LV NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit
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SG Cell Line 4D-Nucleofector® X Kit S
Catalog #: V4XC-3032
For efficient transfection of difficult-to-transfect cell lines, e.g. Ba/F3, Raji, or THP-1, in the 4D-Nucleofector® X Unit (20 µL format).
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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References - X-Vivo™Overview of relevant references
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Instructions - Neural Progenitor Cell System, NHNPInstructions for Proper Use
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TechSheet - Neural Progenitor Cell System, NHNPTechnical Product Information Sheet
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured. -
Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive. -
Terms and ConditionsCD34+ cells BOGO
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Human CD34+ Cells Technical information and instructions for use
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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