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What are the four different types of T cells?

Helper T cells Regulatory T cells Cytotoxic T cells Memory T cells
Role / function Secrete cytokines once stimulated by an antigen Control the immune reactions Activated by cytokines and then bind and kill infected or cancer cells Increase immune response after reactivation by reintroducing pathogens
Markers CD4+ CD4+ CD8+ CD4+ or CD8+, CD45RO
Products CD4+ Helper T cells (negatively selected), ≥ 10 mio. cells CD8+ Cytotoxic T Cells (negatively selected), ≥ 10 mi. cells CD4+ or CD8+ Memory T Cells (negatively selected, ≥ 5 mio. cells 

Learn more  about T cells functions and disorders.

What are the different types of T cell therapy?

T cells are used in different therapeutic approaches:

Cell-based adoptive T-cell therapy: Adoptive T-cell therapy focuses on increasing tumor-specific T cells: Various sources and types of T cells have been used for adoptive therapy including tumor-infiltrating lymphocytes (TILs) expanded ex vivo or peripheral blood T cells genetically engineered ex vivo to either express a cancer-specific T-cell receptor (TCR) or a chimeric antigen receptor (CAR; CAR-T cells) against a cancer epitope. 
Learn more about cell-based immunotherapy 

Tumor-Infiltrating Lymphocyte (TIL) therapy: makes use of T cells found within tumors, which may naturally have T cell receptors that recognize targets on cancer cells.

TCR-engineered T-cell therapy: makes use of genetic engineering of a patient’s T cells to add alternative T-cell receptors that recognize targets on cancer cells.

CAR-T cell therapy: makes use of genetic engineering to endow a patient’s T cells with artificial receptors known as “CARs” which can recognize targets on cancer cells that are invisible to T cell receptors.. 
Learn more about CAR-T

In recent years, cell and gene therapy technologies have been gaining traction across multiple therapeutic areas. Prominent examples include the development of CAR-T cell therapy , i.e., the use of CARs to redirect T lymphocytes towards cancer cells. Although relatively new, CAR-T therapy is showing considerable success for the treatment of hematopoietic malignancies such as acute lymphoblastic leukemia, chronic lymphocytic leukemia, lymphoma, and multiple myeloma.

Activation methods for γδ T cells

Webinar
An automated non-viral method for modifying and expanding T cells
White paper

Applications that use T cells

Isolated human T cells or PBMCs are utilized in various fields of research, including vaccine and drug  development. The ability to use cryopreserved cells and be assured of the same functionality as fresh cells allows more options for cell sources, reproducible results, and the ability to plan experiments with convenience. Learn more on the use of cryopreserved immune cells for experimentation.  


Isolated T cells or PBMCs are commonly used during the drug development process in T cell proliferation assays to determine whether a compound or drug will elicit a T cell response to a foreign substance. Researchers may use T cells to investigate multiple areas in immune-oncology such as Blockage of immune checkpoint inhibition; T cell activation via anti-CD3 or other receptors; Ability of bispecific antibodies to physically engage T cells and tumor cells; Creation of T cells expressing tumor cell-specific T cell receptors (CAR-T).

PBMC based T cell proliferation assay
Protocol
Dendritic cell (DC) and T cell assay from matched PBMCs
Protocol

What are common disorders that affect T cells?

There are some autoimmune diseases and immunodeficiency disorders that affect T cells like
  • Acute lymphocytic leukemia
  • Adult Hodgkin lymphoma
  • Chronic t cell leucemia
  • DiGeorge syndrome
  • HIV
  • SCID ( severe combined immunodeficiency)
  • Job syndrome
  • T cell lymphoma
  • Thymic aplasia
  • Wiskott-Alldrick syndrome 

References

Grens K. The Next Frontier of CAR T-Cell Therapy: Solid Tumors. The Scientist. 2019 Apr

Zhao Z, Chen Y, NM Francisco, Zhang Y, Wu M. The application of CAR-T cell therapy in hematological malignancies: advantages and challenges. Acta Pharmaceutica Sinica B, 2018 Jul; 8(4): 539–551