SF Cell Line 384-well Nucleofector® Kit
SF Cell Line 384-well Nucleofector® Kit
For high-throughput transfection of cell lines, e.g. K-562, Neuro-2A or RAW246.7, when working with the 384-well HT Nucleofector® System.
Select Size
You May Also Need...
Product Overview
The SF Cell Line 384-well Nucleofector® Kit is one of our three kits suited for high-throughput transfection of cell lines when working with the 384-well HT Nucleofector® System.
This kit has been determined to be optimal for transfecting following cell types:
- 293-F (FreeStyle), 32D
- A-20, A2058, A375, A431, A549, AGS, ARPE-19
- BetaTC/6, BxPC-3
- C6, CCRF-CEM, CHO-K1, CHO-S (Invitrogen), Cos-1
- EL4
- FDC-P1
- H9c2 (2-1), HEK293, Hel9217, HepG2, High 5, HL-60, HMC-1-8, HT-1080, HT-29, Hut-78, HUV-EC-C
- IMR-32
- J774A.1
- K-562
- KG-1
- L-428, LNCaP
- MDA-MB-453, MDBK, Molt-4, MV-4-11
- Nalm-6, NB-4, NCI-H1299 [H1299], Neuro-2a [N2a], NG108-15, NS0
- P19, P815, PC-12, PC-3
- Raw 264.7, RBL-1, RBL-2H3, Reh, Rin m5F, RPMI 8226
- S2, S49, SaOS, SF9, SH-SY5Y, SK-BR-3, SK-N-AS, SK-N-MC, SK-N-SH, SK-OV-3, Sp2/0-Ag14, SW480
- T2, T84, TF-1
- U266B1, U-937
- Vero
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 2 x 384 or 10 x 384 reactions.
Request More InfoBenefits
- High transfection efficiencies in difficult-to-transfect cell lines
- Cells maintain functionality post transfection
- Seamless integration into automated liquid handling environments
- Conditions are transferable between 4D-Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System
Applications
- Transfection of low cell numbers down to 2 x 104 cell lines
- High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries
Storage and Content
22.5 mL SF Cell Line Nucleofector® Solution
5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 384-well Nucleocuvette® Plate (20 μL)
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
-
4D-Nucleofector® Protocol for TF-1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for TF-1 -
4D-Nucleofector® Protocol for A-375
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for A-375 -
4D-Nucleofector® Protocol for CCRF-CEM
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for CCRF-CEM -
4D-Nucleofector® Protocol for C6 Glioma
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for C6 Glioma -
4D-Nucleofector® Protocol for L428
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for L428 -
4D-Nucleofector® Protocol for FDC-P1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for FDC-P1 -
4D-Nucleofector® Protocol for CHO-K1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for CHO-K1 -
4D-Nucleofector® Protocol for EL4
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for EL4 -
4D-Nucleofector® Protocol for K562
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for K562 -
4D-Nucleofector® Protocol for HL-60
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for HL-60
