Product Overview
MetaPhor® Agarose is a high resolution agarose that challenges polyacrylamide. MetaPhor® Agarose is an intermediate melting temperature (75°C) agarose with twice the resolution capabilities of the finest-sieving agarose products. For example, a 200 bp DNA fragment can be separated from a 204 bp fragment. Using fast running protocols DNA differing in size by 1% can be resolved in as little as 1.5 hours in a 20 cm long horizontal or vertical gel format. MetaPhor® Agarose gels (2% to 4%) approximate the resolution of polyacrylamide gels (4% to 8%). These agarose gels are ideal for resolving AMPFLPs, STRs, and tri- and tetranucleotide repeats. Ideal for small PCR fragments, MetaPhor® Agarose is the choice agarose for resolving hard to see bands.
Analytical Specifications
Gelling temperature (3%) ≤35°C
Melting temperature (3%) ≤75°C
Gel strength (3%) ≥300 g/cm²
Information about bulk and custom packaging is available through Lonza’s Customer Service Team.
Request More InfoBenefits
- All Lonza Agarose is quality tested to certify performance
- Very fine resolution of small DNA fragments
- High gel strength
- Low EEO
Applications
- Small Fragments
- PCR
Storage and Content
MetaPhor® Agarose 25 g
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
Educational Material
Brochures, White Papers etc.
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SourceBook Section XIII - Protein Separation in Agarose Gels
1. Introduction 2. Buffers for Protein Separation in Agarose 3. Casting Agarose Gels for Protein Separation 4. Preparation and Loading of Protein Samples 5. Optimal Voltage and Electrophoretic Times 6. Detection of Proteins in Agarose Gels 7. Gel Drying and Preservation 8. Processing Agarose Gels Following Electrophoresis 9. Recovering Proteins from Agarose Gels 10. References -
SourceBook Section II - Preparation of Agarose Gels
1. Agarose Selection Guide 2. Agarose and Compatible Techniques 3. Agarose Types 4. Agarose Analytical Specifications 5. Suggested Agarose Concentrations & Dye Migration Information 6. Buffers for Electrophoresis 7. Dissolving Agarose 8. Casting Agarose Gels -
Overview Lonza DNA Markers and Ladders
Select the best DNA Marker for your application -
SourceBook Section IV - Detection and Sizing of DNA in Agarose Gels
1. Guide to Lonza Ladders and Markers 2. Detecting DNA with GelStar® and SYBR® Green Nucleic Acid Gel Stains 3. Detecting DNA with Ethidium Bromide 4. High Sensitivity Detection using the FlashGel® System 5. References -
SourceBook Section III - Loading and Running DNA in Agarose Gels
1. DNA Loading 2. Loading Buffers 3. Optimal Voltage and Electrophoretic Times 4. Fast Running Protocols for High Resolution in MetaPhor® Agarose Gels 5. References -
Agarose Selection Guide
Select the best agarose for your application to minimize opportunity for error, optimize results and even reduce costs.
