For adherent electroporation of primary cells, e.g. neuronal or endothelial cells, when working with the 4D-Nucleofector® Y Unit.
For efficient transfection of specific primary cells, e.g. human ADSCs or MSCs, in the 4D-Nucleofector® X Unit (100 µL format).
For efficient transfection of specific primary cells, e.g. human ADSCs or MSCs, in the 4D-Nucleofector® X Unit (100 µL format).
Cryopreserved ampule of Normal Human Neonatal Dermal Fibroblasts (NHDF-Neo) containing ≥ 500,000 cells
Cryopreserved Human Neonatal Epidermal Keratinocytes, Pooled, ≥ 500,000 cells
Naïve Kupffer cells derived from human liver tissue. 0.2 million cells per vial
Naïve Kupffer cells derived from human liver tissue. 0.5 million cells per vial.
SmGM®-2 Smooth Muscle SingleQuots® Kit, supplements and growth factors required for culture of human smooth muscle cells (basal media sold separately).
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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References - X-Vivo™Overview of relevant references
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Instructions - Neural Progenitor Cell System, NHNPInstructions for Proper Use
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TechSheet - Neural Progenitor Cell System, NHNPTechnical Product Information Sheet
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured. -
Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive. -
Terms and ConditionsCD34+ cells BOGO
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Human CD34+ Cells Technical information and instructions for use
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit