SE Cell Line 4D-Nucleofector™ X Kit L
SE Cell Line 4D-Nucleofector™ X Kit L
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Product Overview
The SE Cell Line 4D-NucleofectorTM X Kit L is one of our three 4D-NucleofectorTM Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL NucleocuvetteTM Vessel format when working with the 4D-NucleofectorTM X Unit.
This kit has been determined to be optimal for transfecting following cell types:
- 3T3-L1, diff., 3T3-L1, undiff.
- A-10, A7r5,
- BHK-21, BJ
- C2C12, CaCo-2, Calu-3, Capan-1, Cos-7
- DU145
- GH3
- HCT116, HeLa, HeLa S3
- IMR-90
- Jurkat (DSMZ), Jurkat E6.1
- KG-1a, L6
- MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MEG-01, MG-63, MRC-5
- NCTC clone 929
- Panc-1
- T/G-HA VSMC, T-47D
- U2OS, U-87 MG
- WI-38
- T84
The respective cell type-specific Optimized Protocols are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 12 or 24 reactions.
Request More InfoBenefits
- High transfection efficiencies in difficult-to-transfect cell lines
- Cells maintain functionality post transfection
- Conditions are transferable between 4D-NucleofectorTM System (including 4D-NucleofectorTM including LV Unit), 96-well ShuttleTM System and 384-well NucleofectorTM System
Applications
- Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells
- Low throughput applications
Storage and Content
2.25 mL SE Cell Line NucleofectorTM Solution
0.5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
24 x 100 µL NucleocuvetteTM Vessel,
24 x Single use pipettes
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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DU 145 Transfection Protocol - 4D-Nucleofector™ Device
Instructions of use for transfection of DU 145 with the SE Cell Line 4D-Nucleofector™ X Kit -
T84 Transfection Protocol - 4D-Nucleofector™ Device
Instructions of use for transfection of T84 with the SE Cell Line 4D-Nucleofector™ X Kit -
IMR-90 Transfection Protocol - 4D-Nucleofector™ Device
Instructions of use for transfection of IMR-90 cells with the SE Cell Line 4D-Nucleofector™ X Kit -
HeLa S3 Transfection Protocol - 4D-Nucleofector™ Device
Instructions of use for transfection of HeLa S3 with the SE Cell Line 4D-Nucleofector™ X Kit -
MRC-5 Transfection Protocol - 4D-Nucleofector™ Device
Instructions of use for transfection of MRC-5 with the SE Cell Line 4D-Nucleofector™ X Kit -
MCF7 Transfection Protocol - 4D-Nucleofector™ Device
Instructions of use for transfection of MCF7 with the SE Cell Line 4D-Nucleofector™ X Kit -
U-87 MG Transfection Protocol - 4D-Nucleofector™ Device
Instructions of use for transfection of U-87 MG cells with the SE Cell Line 4D-Nucleofector™ X Kit -
MG-63 Transfection Protocol - 4D-Nucleofector™ Device
Instructions of use for transfection of MG-63 with the SE Cell Line 4D-Nucleofector™ X Kit -
C2C12 Transfection Protocol - 4D-Nucleofector™ Device
Instructions of use for transfection of C2C12 cells with the SE Cell Line 4D-Nucleofector™ X Kit -
Calu-3 Transfection Protocol - 4D-Nucleofector™ Device
Instructions of use for transfection of Calu-3 with the SE Cell Line 4D-Nucleofector™ X Kit