Human Umbilical Vein Endothelial Cell Nucleofector® Kit
Human Umbilical Vein Endothelial Cell Nucleofector® Kit
For efficient transfection of primary HUVECs in the Nucleofector® 2b System
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Product Overview
The Nucleofector® 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector® Kit is the optimal kit for efficient transfection of primary human umbilical vein endothelial cells (HUVECs).
The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.
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Benefits
- High transfection efficiencies for primary cells
- Cells maintain functionality post transfection
Applications
- Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells
- Low-throughput transfection in single cuvette format
Storage and Content
2.25 mL Nucleofector® Solution for Human Umbilical Vein Endothelial Cells
0.5 mL Supplement 3
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL)
25 Single use pipettes
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
