For efficient transfection of specific primary cells, e.g. human T cells, CD34 or rat neurons, in the 4D-NucleofectorTM X Unit (100 µL format).
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hMSC Human Mesenchymal Stem Cell Osteogenic Differentiation Basal Medium, 170 mL
Cryopreserved ampule of Human Mesenchymal Stem Cells (hMSCs) containing ≥750,000 cells
hMSC Osteogenic Differentiation SingleQuots® Kit
Cryopreserved ampule of Diseased Human Lung Fibroblasts, Idiopathic Pulmonary Fibrosis (DHLF-IPF) containing ≥ 500,000 cells
NoSpinTM HepaRGTM (8.0M cells/vial)
Fully differentiated HepaRGTM cells supplied in a format that does not require centrifugation following thaw. Each ampule contains ≥8.0 million cells.
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Instructions - Articular Chondrocyte Cell System, NHAC-knTechnical instructions for culturing Clonetics™ Human Articular Chondrocytes
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Isolation and Cell Culture of Primary Human ChondrocytesTechnical Reference Guide
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Transfection Protocol for Human Chondrocytes - HT Nucleofector™ DeviceInstructions of use for transfection of human chondrocytes with the P3 Primary Cell HT-Nucleofector™ Kit
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Transfection Protocol for NHAC-kn - 4D-Nucleofector™ DeviceInstructions of use for transfection of normal human articular chondrocytes (knee) with the P3 Primary Cell 4D-Nucleofector™ X Kit
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Transfection Protocol for NHAC-kn - Nucleofector™ I/II/2b Device SeriesInstructions of use for transfection of normal human articular chondrocytes with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)
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Transfection Protocol for NHAC-kn - 96-well Shuttle™ DeviceInstructions of use for transfection of normal human articular chondrocytes (knee) with the P3 Primary Cell 96-well Nucleofector™ Kit
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Webinar – Efficient Transfection of Biologically Relevant Cells in Immunology Research (pdf)Lonza_Webinars_Webinar__Efficient_Transfection_of_Biologically_Relevant_Cells_in_Immunology_Research.pdf
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Monitoring_of hMSC DifferentiationCo-marketed scientific poster with Sistemic Monitoring_of hMSC Differentiation with Histo- or Biochemical Assays and miRNA Expression Analysis
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Myoblasts Differentiation into MyotubesVideo of myoblasts differentiating into multinucleated myobtubes, recorded for 5 days with the CytoSMART™ Lux 10X System, the previous version of the CytoSMART™ 2 System. (Video courtesy of Marco Thurner, Innovacell Biotechnologie AG) -
Supplemental Inst. - HSMM Differentiation to Form MyotubesSupplemental myotube differentiation instructions for ClonticsTM Human Skeletal Muscle Myoblasts