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SAEC – Human Small Airway Epithelial Cells

SAEC – Human Small Airway Epithelial Cells

Catalog #: CC-2547

Cryopreserved ampule of Small Airway Epithelial Cells (SAEC) containing ≥500,000 cells.

 

Interested in reducing lab time? Lonza offers proliferating primary cells in a variety of convenient flask and plate formats. Request more information or quote now.

Do you need a modified version of this cell product, such as different vialing sizes, or isolations from special donors or species? Contact Lonza CellBio Services to discuss custom isolation, expansion, and testing options for your research

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 1’989.00 CHF
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Product Overview

Lonza's Human Small Airway Epithelial Cells (SAEC) are isolated from normal human lung tissue the distal portion of the lung in the 1mm bronchiole area.

Cryopreserved SAECs or Alveolar Cells are guaranteed through 15 population doublings and stain positive for cytokeratin 19.  SAECs or Alveolar Cells labeled CC-2547S are suitable for air-liquid interface studies and are guaranteed for differentiation marked by cilia formation, mucin production, and TEER levels. Cells from various diseased donors are also available. 

All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

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Benefits

  • All cells test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis provided for every cell lot

 

Storage and Content

1 x Cryopreserved ampule SAEC containing ≥500,000 cells

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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • TechSheet - Small Airway Epithelial Cell Systems

    Technical Product Information Guide
  • Instructions - Airway Epithelial Cell Systems

    Instructions for Proper Use and Culture of Airway Epithelial Cells
  • Primary Cells and Media Reference Guide

    A comprehensive collection of Lonza primary cell and media products supporting many research areas.
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Educational Material

Brochures, White Papers etc.

  • Primary Human Airway Cells and Media

    Airway Cell and Media Reference Guide
  • Primary Cells and Media Reference Guide

    A comprehensive collection of Lonza primary cell and media products supporting many research areas.
  • Primary Cells for Airway Research – Infographic

    Infographic showing how Lonza primary cells can aide in your airway research
  • Supporting In Vitro ADMETox with an Advanced Portfolio of Primary Cells and Media

    Reference to Help Get Started on Primary Cell Culture for ADMETox Applications
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Data

Co-culture Experiments with Lonza's Small Airway Epithelial Cells and SAGM™ BulletKit™ Media

Data Courtesy of Furukawa, et al. Lonza's SAEC cells were co-cultured with primary normal mammary epithelial cells, breast cancer cell lines MCF-7 and MDA-MB-231 in Lonza's SAGM™ BulletKit™ Growth Media. Breast cancer cell lines showed differences in morphology and proliferation rates in the presence of small airway epithelial cells. The image above shows phase contrast imaging with a fluorescent (red) overlay of MCF-7 cell line. MCF-7 cell lines survived as tight clusters in SAGM™ Media. When co-cultured with Lonza's primary SAECs, MCF-7 cells demonstrated enhanced growth all the while staying clustered.

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