Product Overview
SeaKem® Gold Agarose is a very high gel strength, low EEO, standard gelling temperature agarose. This Genetic Technology GradeTM Agarose is for rapid resolution of megabase DNA by pulsed field gel electrophoresis (PFGE). Due to its low EEO, the electrophoretic mobility of DNA in SeaKem® Gold Agarose gels is significantly greater than in conventional agarose gels. Run times for PFGE can be decreased by as much as 50% depending upon buffer and agarose concentration. Due to its high gel strength, SeaKem® Gold Agarose forms easy-to-handle gels at low concentrations (as low as 0.5%), which allow for the separation of larger DNA fragments by conventional electrophoresis as well as a decrease in the time needed to separate DNA by PFGE.
Analytical Specifications
Gelling temperature (1.5%) 36°C ±1.5°C
Melting temperature (1.5%) ≥90°C
Gel strength (1.0%) ≥1,800 g/cm²
Gel strength (1.5%) ≥3,500 g/cm²
Information about bulk and custom packaging is available through Lonza’s Customer Service Team.
Benefits
- All Lonza Agarose is quality tested to certify performance
- Highest gel strengh
- Low EEO
- Fast separations of megabase sized DNA fragments using PFGE
Applications
- PFGE
- Large Fragment Electrophoresis
Storage and Content
- SeaKem® Gold Agarose 125 g
