Product Overview
CD14+ monocytes are negatively selected from leukapheresed peripheral blood MNCs using an immunomagnetic labeling system to remove unwanted cell types.
Cells are cryopreserved in Lonza Cryo Media in a cryovial. Cell purity is determined post thaw using a FACS assay. Count and viability is determined using hemacytometer (trypan blue method). Cells are collected from healthy donors following IRB protocols.
Request More InfoBenefits
- Cells are isolated from peripheral blood obtained from a healthy donor
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis (CoA) is provided for each cell lot purchased
Applications
- Generate monocyte-derived M1 and M2 macrophages or dendritic cells
- Dendritic cell differentiation and maturation
- Inflammatory studies
- Toll-like receptor and pattern recognition receptors (PRR)
- Phagocytosis
- Metabolism mechanisms
Storage and Content
1 x Cryopreserved ampule of CD14+ Monocytes containing ≥ 50 million cells