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CompareP3 Primary Cell 4D-Nucleofector® X Kit LCatalog #: V4XP-3024
For efficient transfection of specific primary cells, e.g. human T cells, CD34 or rat neurons, in the 4D-NucleofectorTM X Unit (100 µL format).
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CompareFerric Citrate Agent for Anti-clumping, 1 L bottleCatalog #: BE02-059Q
Ferric Citrate (100x) Agent, 1 L (100x)
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CompareFerric Citrate Agent 100 ml bottleCatalog #: BE02-059E
Ferric Citrate (100x) Agent, 100ml
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CompareFerric Citrate Agent 500 ml bottleCatalog #: BE02-059FFerric Citrate (100x) Agent, 500mlSales unit: each
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CompareAnti-clumping Agent A, 100 mlCatalog #: BE02-058EAnti-clumping Agent A, 100 ml
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CompareRat Neonatal Retinal CellsCatalog #: R-RET-508
Cryopreserved ampule of Rat Neonatal Retinal Cells containing ≥200,000 cells
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CompareP4 Primary Cell 4D-Nucleofector® X Kit SCatalog #: V4XP-4032
For efficient transfection of specific primary cells, e.g. mouse B-cells or mouse immature DCs, in the 4D-Nucleofector® X Unit (20 µL format).
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CompareP5 Primary Cell 4D-Nucleofector® X Kit SCatalog #: V4XP-5032
For efficient transfection of specific primary cells, e.g. HUVEC, in the 4D-Nucleofector® X Unit (20 µL format).
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CompareP2 Primary Cell 4D-Nucleofector® X Kit SCatalog #: V4XP-2032
For efficient transfection of specific primary cells, e.g. mouse macrophages and NHDF, in the 4D-Nucleofector® X Unit (20 µL format).
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CompareSG Cell Line 4D-Nucleofector® X Kit SCatalog #: V4XC-3032
For efficient transfection of difficult-to-transfect cell lines, e.g. Ba/F3, Raji, or THP-1, in the 4D-Nucleofector® X Unit (20 µL format).
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Terms and ConditionsCD34+ cells BOGO
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Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive. -
CD34+ Cells for Cell and Gene Therapy Process DevelopmentIn this interview Dr. Manon Destalminil, Bioprocessing Project Leader at CellProthera, discusses the advantages of using research-grade CD34+ cells for cell and gene therapy process development. Please register to read.
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Human CD34+ Cells Technical information and instructions for use
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - 4D-Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 4D-Nucleofector™ X Kit
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - Nucleofector™ I/II/2b Device SeriesInstructions of use for transfection of human CD34+ cells with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured. -
Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf