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SeaKem® GTG® AgaroseCatalog #: 50075
SeaKem® GTG® Agarose, 1 kg, ensures reliable digestion and ligation of recovered DNA fragments.
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SeaKem® GTG® AgaroseCatalog #: 50071
SeaKem® GTG® Agarose, 25 g, ensures reliable digestion and ligation of recovered DNA fragments.
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TheraPEAK® SfAAV® MediumCatalog #: BP12-945Q
TheraPEAK® SfAAV® Medium for AAV production in SF9 cells, chemically defined, hydrolysate free, 1 L
Sales unit: Bottle -
SeaKem® Gold AgaroseCatalog #: 50152
SeaKem® Gold Agarose, 25 g, ideal for separation of large DNA fragments using low concentration gels or PFGE.
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SeaKem® Gold AgaroseCatalog #: 50150
SeaKem® Gold Agarose, 125 g, ideal for separation of large DNA fragments using low concentration gels or PFGE.
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D-HPBMC - Diseased Human Peripherial Blood Mononuclear Cells (Psoriasis)Catalog #: 00219738Limited Edition Peripherial Blood Mononuclear from Psoriasis DonorSales unit: Ampule
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P-HEK - Diseased Human Epidermal Keratinocytes (Non-Lesion)Catalog #: 00219735Limited Edition Epidermal Keratinocytes from Psoriasis Donor (Non-Lesion Site Derived)Sales unit: Ampule
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P-HDF - Diseased Human Dermal Fibroblast (Lesion)Catalog #: 00219736Limited Edition Dermal Fibroblasts from Psoriasis Donor (Lesion Site Derived)Sales unit: Ampule
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TheraPEAK® LV Nucleocuvette® Cartridge, weldable (5 pcs)Catalog #: G4LN-7520W
For use in combination with TheraPEAK® Nucleofector® Solution Sets, supporting the use of the 4D-Nucleofector® LV Unit in a GMP environment.
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TheraPEAK® 4D-Nucleofector® LV Reservoirs, weldable (5 pcs)Catalog #: G4LR-1501WFor use in combination with TheraPEAK® Nucleocuvette® Cartridges, supporting the use of the 4D-Nucleofector® LV Unit in a GMP environment.Sales unit: items
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Terms and ConditionsCD34+ cells BOGO
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Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive.
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CD34+ Cells for Cell and Gene Therapy Process DevelopmentIn this interview Dr. Manon Destalminil, Bioprocessing Project Leader at CellProthera, discusses the advantages of using research-grade CD34+ cells for cell and gene therapy process development. Please register to read.
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured.
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Human CD34+ Cells Technical information and instructions for use
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - 4D-Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 4D-Nucleofector™ X Kit
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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Transfection Protocol for Human CD34+ Cells - Nucleofector™ I/II/2b Device SeriesInstructions of use for transfection of human CD34+ cells with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)
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