For efficient transfection of difficult-to-transfect cell lines, e.g. Jurkat or MCF-7, in the 4D-Nucleofector® X Unit (20 µL format).
Maintenance medium for use in adipogenic differentiation of mesenchymal stem cells
Induction Medium for adipogenic differentiation of mesenchymal stem cells
TheraPEAK® ProFreeze® Freezing Medium, chemically defined, non-animal origin, 100 mL (2x)
X-VIVO® 15, with L-Glutamine, gentamicin, recombinant transferrin, and phenol red, xenofree, 1 L
X-VIVO® 15, Serum-free hematopoietic cell medium, with L-Glutamine, gentamicin and phenol red, xenofree, 500mL
Kinetic Chromogenic LAL Assay for endotoxin detection for 192 tests, assay sensitivity: 0.005 - 50 EU/mL
Culture system containing SABMTM Basal Medium and SAGMTM SingleQuots® supplements required for growth of Small Airway Epithelial cells
For adherent electroporation of primary cells, e.g. fibroblasts, when working with the 4D-Nucleofector® Y Unit.
Basal media required for growth of endothelial cells, phenol red free, 500 mL (supplements and growth factors sold separately)
-
Terms and ConditionsCD34+ cells BOGO
-
Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive. -
CD34+ Cells for Cell and Gene Therapy Process DevelopmentIn this interview Dr. Manon Destalminil, Bioprocessing Project Leader at CellProthera, discusses the advantages of using research-grade CD34+ cells for cell and gene therapy process development. Please register to read.
-
Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured. -
Human CD34+ Cells Technical information and instructions for use
-
Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
-
Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
-
Transfection Protocol for Human CD34+ Cells - 4D-Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 4D-Nucleofector™ X Kit
-
Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
-
Transfection Protocol for Human CD34+ Cells - Nucleofector™ I/II/2b Device SeriesInstructions of use for transfection of human CD34+ cells with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)