Stellate Cells, Human Cryopreserved, 0.2 Million Cells
Stellate Cells, Human Cryopreserved, 0.2 Million Cells
Low passage stellate cells dervied from human liver tissue containing 200,000 cells/vial.
Do you need a modified version of this cell product, such as different vialing sizes, or isolations from special donors or species? Contact Lonza CellBio Services to discuss custom isolation, expansion, and testing options for your research
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Product Overview
Stellate cells are a resident cell type of the liver primarily functioning to store retinoids. In response to liver damage, stellate cells rapidly lose the stored retinoids and differentiate into a proliferating fibroblast-like cell that begins depositing collagen matrix. This activity causes buildup of collagen in the liver eventually leading to cirrhosis.
Stellate cells can be isolated from liver tissue, enriched, and placed into cell culture. When placed into culture, stellate cells begin to immediately differentiate into the activated state, characterized by high expression of alpha-smooth muscle actin, a-SMA.
Human Stellate Cells are stellate cells enriched from human liver isolations and grown to >80% confluency and then cryopreserved.
Human Stellate Growth Medium (Catalog MST-500BK) is available to support optimal culture health and growth characteristics of stellate cells.
Characterization performed:
- Post-thaw plating to observe characteristic stellate morphology.
- Flow cytometry using antibodies for Vimentin Immunocytochemistry for a-SMA
Benefits
Low passage for high activation potential
Characterized for alpha-SMA
Applications
- ADMETox
- Disease research
Storage and Content
- Cryopreserved Cells, 200,000 viable cells
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
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Instructions for Use - Hepatocyte and Non-Parenchymal Cell (NPC) 2D Co-Culture
Protocol for 2D co-culture of human hepatocytes and non-parenchymal cells -
Hepatocytes and Non-parenchymal Cells - Development and Optimization of a Comprehensive Co-Culture
Tech Note detailing the development of a co-culture protocol for Heps and NPCs -
Instructions - Cryopreserved Human Stellate Cells
Technical Instructions for culturing human stellate cells -
3D Spheroid Primary Human Hepatocyte and Non-Parenchymal Cell (NPC) Co-Culture - Instructions for use
