P3 Primary Cell 384-well Nucleofector® Kit
P3 Primary Cell 384-well Nucleofector® Kit
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Product Overview
The P3 Primary Cell 384-well Nucleofector® Kit is one of our five kits suited for high-throughput transfection of primary cells when working with the 384-well HT Nucleofector® System.
This kit has been determined to be optimal for transfecting following cell types:
- B cell (human)
- CD34+ cell (human)
- Chondrocyte (human)
- DC (human)
- DC (mouse), mature
- ES (human), H9
- ES (mouse)
- Hepatocyte (human)
- HMEC (human)
- Keratinocyte (human)
- Macrophage (human)
- Monocyte (human)
- Neuron (rat), brain Hi/Cx
- NHBE (human)
- RPE (human)
- T cell (human), stim.
- T cell (mouse), unstim.
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 2 x 384 or 10 x 384 reactions.
Request More InfoBenefits
- High transfection efficiencies for primary cells
- Cells maintain functionality post transfection
- Seamless integration into automated liquid handling environments
- Conditions are transferable between 4D-Nucleofector® System, 4D-Nucleofector® 96-well Unit and 384-well Nucleofector® System
Applications
- Transfection of low cell numbers down to 2 x 104 primary cells
- High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries
Storage and Content
22.5 mL P3 Primary Cell Nucleofector® Solution
5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 384-well Nucleocuvette® Plate (20 μl)
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ Device
Instructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit -
Transfection Protocol for Rat Neurons - HT Nucleofector™ Device
Instructions of use for transfection of rat hippocampal/cortical neurons with the P3 Primary Primary Cell HT-Nucleofector™ Kit -
Transfection Protocol for Human Chondrocytes - HT Nucleofector™ Device
Instructions of use for transfection of human chondrocytes with the P3 Primary Cell HT-Nucleofector™ Kit -
Transfection Protocol for Human SC (H9) - HT Nucleofector™ Device
Instructions of use for transfection of human stem cells (H9) with the P3 Primary Cells HT-Nucleofector™ Kit -
Transfection Protocol for NHEK (Lonza) - HT Nucleofector™ Device
Instructions of use for transfection of normal human epidermal keratinocytes with the P3 Primary Cell HT-Nucleofector™ Kit -
Transfection Protocol for Human Hepatocytes - HT Nucleofector™ Device
Instructions of use for transfection of human hepatocytes with the P3 Primary Cell HT-Nucleofector™ Kit -
Basic Transfection Protocol for Human Stem Cells - HT Nucleofector™ Device
Instructions of use for optimizing transfection conditions of human stem cells with the P3 and P4 Primary Cell HT Nucleofector™ Kit -
Basic Transfection Protocol for Epithelial Cells - HT Nucleofector™ Device
Instructions of use for transfection of primary mammalian epithelial cells with the P1 or P3 Primary Cell HT-Nucleofector™ Kit -
Basic Transfection Protocol for Fibroblasts - HT Nucleofector™ Device
Instructions of use for transfection of primary mammalian fibroblast cells with the P2 or P3 Primary Cell HT-Nucleofector™ Kit -
Basic Transfection Protocol for Neural Cells - HT Nucleofector™ Device
Instructions of use for transfection of primary mammalian neurons with the P3 Primary Cell HT Nucleofector™ Kit
